High-throughput verification of transcriptional starting sites by Deep-RACE
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- BTN A 000113066 O 12907a
Final published version, 465 KB, PDF document
We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep–rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes and is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.
Original language | English |
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Journal | BioTechniques |
Volume | 46 |
Issue number | 2 |
Pages (from-to) | 130-132 |
Number of pages | 3 |
ISSN | 0736-6205 |
DOIs | |
Publication status | Published - 2009 |
- Faculty of Science - promoters, transcription start sites, high throughput, RACE, short-reads sequencing
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ID: 17880276