High-throughput verification of transcriptional starting sites by Deep-RACE
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High-throughput verification of transcriptional starting sites by Deep-RACE. / Olivarius, Signe; Plessy, Charles; Carninci, Piero.
In: BioTechniques, Vol. 46, No. 2, 2009, p. 130-132.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - High-throughput verification of transcriptional starting sites by Deep-RACE
AU - Olivarius, Signe
AU - Plessy, Charles
AU - Carninci, Piero
N1 - Paper id:: 19317658
PY - 2009
Y1 - 2009
N2 - We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep–rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes and is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.
AB - We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep–rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes and is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.
KW - Faculty of Science
KW - promoters
KW - transcription start sites
KW - high throughput
KW - RACE
KW - short-reads sequencing
U2 - 10.2144/000113066
DO - 10.2144/000113066
M3 - Journal article
C2 - 19317658
VL - 46
SP - 130
EP - 132
JO - BioTechniques
JF - BioTechniques
SN - 0736-6205
IS - 2
ER -
ID: 17880276