Evaluation of immunofluorescence microscopy and enzyme-linked immunosorbent assay in detection of Cryptosporidium and Giardia infections in asymptomatic dogs
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Evaluation of immunofluorescence microscopy and enzyme-linked immunosorbent assay in detection of Cryptosporidium and Giardia infections in asymptomatic dogs. / Rimhanen-Finne, R.; Enemark, Heidi Larsen; Kolehmainen, J.; Toropainen, P.; Hänninen, M.L.
In: Veterinary Parasitology, Vol. 145, No. 3-4, 2007, p. 345-348.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Evaluation of immunofluorescence microscopy and enzyme-linked immunosorbent assay in detection of Cryptosporidium and Giardia infections in asymptomatic dogs
AU - Rimhanen-Finne, R.
AU - Enemark, Heidi Larsen
AU - Kolehmainen, J.
AU - Toropainen, P.
AU - Hänninen, M.L.
PY - 2007
Y1 - 2007
N2 - The performance of immunoflourscence microscopy (IF) and enzyme-linked immunosorbent assay (ELISA) in canine feces was evaluated. IF and Cryptosporidium ELISA detected 105 oocysts/g, while the detection limit for Guardia ELISA was 104 cysts/g. The Cryptosporidium ELISA showed 94% specificity but only 71% sensitivity. The Guardia ELISA correlated well with IF (sensitivity 100%, specificity 96%) and was capable of detecting animal specific Guardia duodenalis genotypes.
AB - The performance of immunoflourscence microscopy (IF) and enzyme-linked immunosorbent assay (ELISA) in canine feces was evaluated. IF and Cryptosporidium ELISA detected 105 oocysts/g, while the detection limit for Guardia ELISA was 104 cysts/g. The Cryptosporidium ELISA showed 94% specificity but only 71% sensitivity. The Guardia ELISA correlated well with IF (sensitivity 100%, specificity 96%) and was capable of detecting animal specific Guardia duodenalis genotypes.
KW - Former LIFE faculty
KW - Cryptosporidium, Guardia, Dog, IF, ELISA
U2 - 10.1016/j.vetpar.2007.01.008
DO - 10.1016/j.vetpar.2007.01.008
M3 - Journal article
C2 - 17320291
VL - 145
SP - 345
EP - 348
JO - Veterinary Parasitology
JF - Veterinary Parasitology
SN - 0304-4017
IS - 3-4
ER -
ID: 8073058