Hydrolysis of Toxic Natural Glucosides Catalyzed by Cyclodextrin Dicyanohydrins
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The hydrolysis of toxic 7-hydroxycoumarin glucosides and other aryl and alkyl glucosides, catalyzed by modified a- and ß-cyclodextrin dicyanohydrins, was investigated using different UV, redox, or HPAEC detection assays. The catalyzed reactions all followed Michaelis-Menten kinetics, and an impressive rate increase of up to 7569 (kcat/kuncat) was found for the hydroxycoumarin glucoside substrate 4-MUGP. Good and moderate degrees of catalysis (kcat/kuncat) of up to 1259 were found for the natural glucosides phloridzin and skimmin. By using a newly developed catechol detection UV-assay, a weak degree of catalysis was also found for the toxic hydroxycoumarin esculin. A novel synthesized diaminomethyl ß-cyclodextrin showed a weak catalysis of p-nitrophenyl ß-D-glucopyranoside hydrolysis.
Bidragets oversatte titel | Hydrolyse af Naturligt Forekommende Toksiske Glukosider Katalyseret af Cyclodextrin Dicyanohydriner |
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Originalsprog | Engelsk |
Tidsskrift | European Journal of Organic Chemistry |
Sider (fra-til) | 745-752 |
Antal sider | 8 |
ISSN | 1434-193X |
DOI | |
Status | Udgivet - 2008 |
- Det Natur- og Biovidenskabelige Fakultet - Kunstigt Enzym, Supramolekylær Kemi, Glykosidase
Forskningsområder
ID: 1795623