Inhibition of α-glucosidase activity by selected edible seaweeds and fucoxanthin
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Inhibition of α-glucosidase activity by selected edible seaweeds and fucoxanthin. / Zaharudin, Nazikussabah Binti; Stærk, Dan; Dragsted, Lars Ove.
In: Food Chemistry, Vol. 270, 2019, p. 481-486.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Inhibition of α-glucosidase activity by selected edible seaweeds and fucoxanthin
AU - Zaharudin, Nazikussabah Binti
AU - Stærk, Dan
AU - Dragsted, Lars Ove
N1 - CURIS 2019 NEXS 001
PY - 2019
Y1 - 2019
N2 - A 5 mg/mL solution of water, methanol and acetone extracts of seaweeds were used for α-glucosidase inhibition assay hyphenated with high performance liquid chromatography-mass spectrometry (HPLC-HRMS). The results showed acetone extracts of Undaria pinnatifida has the strongest inhibitory effect against α-glucosidase activity with IC50 0.08 ± 0.002 mg/mL. The active compound found in Undaria pinnatifida was identified as fucoxanthin. Analytical standard sample of fucoxanthin significantly inhibited α-glucosidase with IC50 value 0.047 ± 0.001 mg/mL. An inhibition kinetics study indicates that fucoxanthin is showing mixed-type inhibition. These results suggest that Undaria pinnatifida has a potential to inhibit α-glucosidase and may be used as a bioactive food ingredient for glycaemic control.
AB - A 5 mg/mL solution of water, methanol and acetone extracts of seaweeds were used for α-glucosidase inhibition assay hyphenated with high performance liquid chromatography-mass spectrometry (HPLC-HRMS). The results showed acetone extracts of Undaria pinnatifida has the strongest inhibitory effect against α-glucosidase activity with IC50 0.08 ± 0.002 mg/mL. The active compound found in Undaria pinnatifida was identified as fucoxanthin. Analytical standard sample of fucoxanthin significantly inhibited α-glucosidase with IC50 value 0.047 ± 0.001 mg/mL. An inhibition kinetics study indicates that fucoxanthin is showing mixed-type inhibition. These results suggest that Undaria pinnatifida has a potential to inhibit α-glucosidase and may be used as a bioactive food ingredient for glycaemic control.
KW - Faculty of Science
KW - Seaweed
KW - Glycaemic control
KW - Hyperglycaemia
KW - α-Glucosidase
KW - Fucoxanthin
U2 - 10.1016/j.foodchem.2018.07.142
DO - 10.1016/j.foodchem.2018.07.142
M3 - Journal article
C2 - 30174076
VL - 270
SP - 481
EP - 486
JO - Food Chemistry
JF - Food Chemistry
SN - 0308-8146
ER -
ID: 201905367