Human muscular mitochondrial fusion in athletes during exercise
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Human muscular mitochondrial fusion in athletes during exercise. / Huertas, Jesús R; Ruiz-Ojeda, Francisco Javier; Plaza-Díaz, Julio; Nordsborg, Nikolai Baastrup; Martín-Albo, Jesús; Rueda-Robles, Ascensión; Casuso, Rafael A.
In: F A S E B Journal, Vol. 33, No. 11, 2019, p. 12087-12098.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Human muscular mitochondrial fusion in athletes during exercise
AU - Huertas, Jesús R
AU - Ruiz-Ojeda, Francisco Javier
AU - Plaza-Díaz, Julio
AU - Nordsborg, Nikolai Baastrup
AU - Martín-Albo, Jesús
AU - Rueda-Robles, Ascensión
AU - Casuso, Rafael A
N1 - CURIS 2019 NEXS 260
PY - 2019
Y1 - 2019
N2 - The main objective of this work was to investigate whether mitochondrial fusion occurs in the skeletal muscle of well-trained athletes in response to high-intensity exercise. Well-trained swimmers (n = 9) performed a duration-matched sprint interval training (SIT) and high-intensity high-volume training (HIHVT) session on separate days. Muscle samples from M. triceps brachii were taken before, immediately after, and 3 h after the training sessions. Transmission electron microscopy (TEM) was applied to assess mitochondrial morphology. Moreover, expression of genes coding for regulators of mitochondrial fusion and fission were assessed by real-time quantitative PCR. In addition, mitofusin (MFN)2 and optic atrophy 1 (OPA1) were quantified by Western blot analysis. TEM analyses showed that mitochondrial morphology remained altered for 3 h after HIHVT, whereas SIT-induced changes were only evident immediately after exercise. Only SIT increased MFN1 and MFN2 mRNA expression, whereas SIT and HIHVT both increased MFN2 protein content 3 h after exercise. Notably, only HIHVT increased OPA1 protein content. Mitochondrial morphologic changes that suggest fusion occurs in well-adapted athletes during exercise. However, HIHVT appears as a more robust inducer of mitochondrial fusion events than SIT. Indeed, SIT induces a rapid and transient change in mitochondrial morphology.
AB - The main objective of this work was to investigate whether mitochondrial fusion occurs in the skeletal muscle of well-trained athletes in response to high-intensity exercise. Well-trained swimmers (n = 9) performed a duration-matched sprint interval training (SIT) and high-intensity high-volume training (HIHVT) session on separate days. Muscle samples from M. triceps brachii were taken before, immediately after, and 3 h after the training sessions. Transmission electron microscopy (TEM) was applied to assess mitochondrial morphology. Moreover, expression of genes coding for regulators of mitochondrial fusion and fission were assessed by real-time quantitative PCR. In addition, mitofusin (MFN)2 and optic atrophy 1 (OPA1) were quantified by Western blot analysis. TEM analyses showed that mitochondrial morphology remained altered for 3 h after HIHVT, whereas SIT-induced changes were only evident immediately after exercise. Only SIT increased MFN1 and MFN2 mRNA expression, whereas SIT and HIHVT both increased MFN2 protein content 3 h after exercise. Notably, only HIHVT increased OPA1 protein content. Mitochondrial morphologic changes that suggest fusion occurs in well-adapted athletes during exercise. However, HIHVT appears as a more robust inducer of mitochondrial fusion events than SIT. Indeed, SIT induces a rapid and transient change in mitochondrial morphology.
KW - Faculty of Science
KW - Physical activity
KW - Mitochondrial fission
KW - Skeletal muscle
KW - Mitofusin 2
U2 - 10.1096/fj.201900365RR
DO - 10.1096/fj.201900365RR
M3 - Journal article
C2 - 31398297
VL - 33
SP - 12087
EP - 12098
JO - F A S E B Journal
JF - F A S E B Journal
SN - 0892-6638
IS - 11
ER -
ID: 225954820