Prevention of H2O2-induced oxidative stress in Chang liver cells by 4-hydroxybenzyl-chitooligomers
Research output: Contribution to journal › Journal article › Research › peer-review
In this study, a bioactive derivative of chitooligomers (1.0–3.0 kDa), 4-hydroxybenzyl-COS (HB-COS), was synthesized to enhance antioxidant activity. Hence, HB-COS was evaluated for its capabilities against H2O2-induced oxidative stress in human Chang liver cells. It was found that HB-COS possessed the free radical scavenging activity via decreasing the intracellular reactive oxygen species production. Furthermore, HB-COS significantly reduced the oxidation of DNA in a dose-dependent manner. Notably, HB-COS treatment upregulated the gene and protein expressions of antioxidative enzymes and thereby enhancing the intracellular antioxidant mechanisms. In addition, HB-COS treatment caused a remarkable blockade on degradation of inhibitory kappa B alpha (IκB-α) protein and translocation of nuclear factor kappa B (NF-κB). The current study demonstrated that HB-COS effectively attenuated hydrogen peroxide-induced oxidative stress in Chang liver cells by increasing levels of antioxidant enzymes and inhibiting reactive oxygen species generation, DNA oxidation and the NF-κB signaling pathway.
Original language | English |
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Journal | Carbohydrate Polymers |
Volume | 103 |
Pages (from-to) | 502-509 |
ISSN | 0144-8617 |
DOIs | |
Publication status | Published - 27 Dec 2013 |
Externally published | Yes |
- Faculty of Science - Chang liver cells, 4-Hydroxybenzyl-chitooligomers (HB-COS), Free radical scavenging, Antioxidant enzymes, Nuclear factor kappa B (NF-κB)
Research areas
ID: 311345666