Synthesis of structured triacylglycerols containing caproic acid by lipase-catalyzed acidolysis: Optimization by response surface methodology

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Synthesis of structured triacylglycerols containing caproic acid by lipase-catalyzed acidolysis : Optimization by response surface methodology. / Zhou, D.; Xu, X.; Mu, Huiling; Høy, Carl-Erik; Adler-Nissen, Jens.

In: Journal of Agricultural and Food Chemistry, Vol. 49, No. 12, 01.01.2001, p. 5771-5777.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhou, D, Xu, X, Mu, H, Høy, C-E & Adler-Nissen, J 2001, 'Synthesis of structured triacylglycerols containing caproic acid by lipase-catalyzed acidolysis: Optimization by response surface methodology', Journal of Agricultural and Food Chemistry, vol. 49, no. 12, pp. 5771-5777. https://doi.org/10.1021/jf0103020

APA

Zhou, D., Xu, X., Mu, H., Høy, C-E., & Adler-Nissen, J. (2001). Synthesis of structured triacylglycerols containing caproic acid by lipase-catalyzed acidolysis: Optimization by response surface methodology. Journal of Agricultural and Food Chemistry, 49(12), 5771-5777. https://doi.org/10.1021/jf0103020

Vancouver

Zhou D, Xu X, Mu H, Høy C-E, Adler-Nissen J. Synthesis of structured triacylglycerols containing caproic acid by lipase-catalyzed acidolysis: Optimization by response surface methodology. Journal of Agricultural and Food Chemistry. 2001 Jan 1;49(12):5771-5777. https://doi.org/10.1021/jf0103020

Author

Zhou, D. ; Xu, X. ; Mu, Huiling ; Høy, Carl-Erik ; Adler-Nissen, Jens. / Synthesis of structured triacylglycerols containing caproic acid by lipase-catalyzed acidolysis : Optimization by response surface methodology. In: Journal of Agricultural and Food Chemistry. 2001 ; Vol. 49, No. 12. pp. 5771-5777.

Bibtex

@article{f0a511397c714c9cb3486d0d8cf0fd16,
title = "Synthesis of structured triacylglycerols containing caproic acid by lipase-catalyzed acidolysis: Optimization by response surface methodology",
abstract = "Production in a batch reactor with a solvent-free system of structured triacylglycerols containing short-chain fatty acids by Lipozyme RM IM-catalyzed acidolysis between rapeseed oil and caproic acid was optimized using response surface methodology (RSM). Reaction time (t ), substrate ratio (S ), enzyme load (E , based on substrate), water content (W , based on enzyme), and reaction temperature (T ), the five most important parameters for the reaction, were chosen for the optimization. The range of each parameter was selected as follows: t = 5-17 h; E = 6-14 wt %; T = 45-65°C; S = 2-6 mol/mol; and W = 2-12 wt %. The biocatalyst was Lipozyme RM IM, in which Rhizomucor miehei lipase is immobilized on a resin. The incorporation of caproic acid into rapeseed oil was the main monitoring response. In addition, the contents of mono-incorporated structured triacylglycerols and di-incorporated structured triacylglycerols were also evaluated. The optimal reaction conditions for the incorporation of caproic acid and the content of di-incorporated structured triacylglycerols were as follows: t = 17 h; S = 5; E = 14 wt %; W = 10 wt %; T = 65°C. At these conditions, products with 55 mol % incorporation of caproic acid and 55 mol % di-incorporated structured triacylglycerols were obtained.",
author = "D. Zhou and X. Xu and Huiling Mu and Carl-Erik H{\o}y and Jens Adler-Nissen",
year = "2001",
month = jan,
day = "1",
doi = "10.1021/jf0103020",
language = "English",
volume = "49",
pages = "5771--5777",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "12",

}

RIS

TY - JOUR

T1 - Synthesis of structured triacylglycerols containing caproic acid by lipase-catalyzed acidolysis

T2 - Optimization by response surface methodology

AU - Zhou, D.

AU - Xu, X.

AU - Mu, Huiling

AU - Høy, Carl-Erik

AU - Adler-Nissen, Jens

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Production in a batch reactor with a solvent-free system of structured triacylglycerols containing short-chain fatty acids by Lipozyme RM IM-catalyzed acidolysis between rapeseed oil and caproic acid was optimized using response surface methodology (RSM). Reaction time (t ), substrate ratio (S ), enzyme load (E , based on substrate), water content (W , based on enzyme), and reaction temperature (T ), the five most important parameters for the reaction, were chosen for the optimization. The range of each parameter was selected as follows: t = 5-17 h; E = 6-14 wt %; T = 45-65°C; S = 2-6 mol/mol; and W = 2-12 wt %. The biocatalyst was Lipozyme RM IM, in which Rhizomucor miehei lipase is immobilized on a resin. The incorporation of caproic acid into rapeseed oil was the main monitoring response. In addition, the contents of mono-incorporated structured triacylglycerols and di-incorporated structured triacylglycerols were also evaluated. The optimal reaction conditions for the incorporation of caproic acid and the content of di-incorporated structured triacylglycerols were as follows: t = 17 h; S = 5; E = 14 wt %; W = 10 wt %; T = 65°C. At these conditions, products with 55 mol % incorporation of caproic acid and 55 mol % di-incorporated structured triacylglycerols were obtained.

AB - Production in a batch reactor with a solvent-free system of structured triacylglycerols containing short-chain fatty acids by Lipozyme RM IM-catalyzed acidolysis between rapeseed oil and caproic acid was optimized using response surface methodology (RSM). Reaction time (t ), substrate ratio (S ), enzyme load (E , based on substrate), water content (W , based on enzyme), and reaction temperature (T ), the five most important parameters for the reaction, were chosen for the optimization. The range of each parameter was selected as follows: t = 5-17 h; E = 6-14 wt %; T = 45-65°C; S = 2-6 mol/mol; and W = 2-12 wt %. The biocatalyst was Lipozyme RM IM, in which Rhizomucor miehei lipase is immobilized on a resin. The incorporation of caproic acid into rapeseed oil was the main monitoring response. In addition, the contents of mono-incorporated structured triacylglycerols and di-incorporated structured triacylglycerols were also evaluated. The optimal reaction conditions for the incorporation of caproic acid and the content of di-incorporated structured triacylglycerols were as follows: t = 17 h; S = 5; E = 14 wt %; W = 10 wt %; T = 65°C. At these conditions, products with 55 mol % incorporation of caproic acid and 55 mol % di-incorporated structured triacylglycerols were obtained.

UR - http://www.scopus.com/inward/record.url?scp=0035543013&partnerID=8YFLogxK

U2 - 10.1021/jf0103020

DO - 10.1021/jf0103020

M3 - Journal article

AN - SCOPUS:0035543013

VL - 49

SP - 5771

EP - 5777

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 12

ER -

ID: 45573346