Methods for histochemical demonstration of vascular structures at the muscle-bone interface from cryostate sections of demineralized tissue
Research output: Contribution to journal › Journal article › Research › peer-review
In tissue decalcified with MgNa2EDTA at a neutral pH activity for ATPase can used be for demonstration of the vascular structures at the muscle-bone interface. The GOMORI method for alkaline phosphatase is only of value, when fresh unfixed tissue is to be examined. The azo-dye method for alkaline phosphatase failed to give satisfactory results, and so did the alpha-amylase PAS method. 5'-nucleotidase activity is present in both capillaries and in cells lining the surfaces of bones, while larger blood vessels are poorly stained.
| Original language | English |
|---|---|
| Journal | Acta Histochemica |
| Volume | 68 |
| Issue number | 1 |
| Pages (from-to) | 111-6 |
| Number of pages | 5 |
| ISSN | 0065-1281 |
| Publication status | Published - 1981 |
Bibliographical note
Keywords: 5'-Nucleotidase; Adenosine Triphosphatases; Alkaline Phosphatase; Amylases; Animals; Bone and Bones; Capillaries; Histocytochemistry; Muscles; Nucleotidases; Rats; Staining and Labeling; alpha-Amylases
ID: 10208912