Methods for histochemical demonstration of vascular structures at the muscle-bone interface from cryostate sections of demineralized tissue
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Methods for histochemical demonstration of vascular structures at the muscle-bone interface from cryostate sections of demineralized tissue. / Kirkeby, S.
In: Acta Histochemica, Vol. 68, No. 1, 1981, p. 111-6.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Methods for histochemical demonstration of vascular structures at the muscle-bone interface from cryostate sections of demineralized tissue
AU - Kirkeby, S
N1 - Keywords: 5'-Nucleotidase; Adenosine Triphosphatases; Alkaline Phosphatase; Amylases; Animals; Bone and Bones; Capillaries; Histocytochemistry; Muscles; Nucleotidases; Rats; Staining and Labeling; alpha-Amylases
PY - 1981
Y1 - 1981
N2 - In tissue decalcified with MgNa2EDTA at a neutral pH activity for ATPase can used be for demonstration of the vascular structures at the muscle-bone interface. The GOMORI method for alkaline phosphatase is only of value, when fresh unfixed tissue is to be examined. The azo-dye method for alkaline phosphatase failed to give satisfactory results, and so did the alpha-amylase PAS method. 5'-nucleotidase activity is present in both capillaries and in cells lining the surfaces of bones, while larger blood vessels are poorly stained.
AB - In tissue decalcified with MgNa2EDTA at a neutral pH activity for ATPase can used be for demonstration of the vascular structures at the muscle-bone interface. The GOMORI method for alkaline phosphatase is only of value, when fresh unfixed tissue is to be examined. The azo-dye method for alkaline phosphatase failed to give satisfactory results, and so did the alpha-amylase PAS method. 5'-nucleotidase activity is present in both capillaries and in cells lining the surfaces of bones, while larger blood vessels are poorly stained.
M3 - Journal article
C2 - 6167124
VL - 68
SP - 111
EP - 116
JO - Acta Histochemica
JF - Acta Histochemica
SN - 0065-1281
IS - 1
ER -
ID: 10208912