Use of a Lariat Capping Ribozyme to Study Cap Function In Vivo

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

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Use of a Lariat Capping Ribozyme to Study Cap Function In Vivo. / Pietschmann, Max; Tempel, Gregor; Halladjian, Maral; Krogh, Nicolai; Nielsen, Henrik.

Ribozymes: Methods and Protocols. Vol. 2167 Humana Press, 2021. p. 271-285 (Methods in Molecular Biology, Vol. 2167).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Pietschmann, M, Tempel, G, Halladjian, M, Krogh, N & Nielsen, H 2021, Use of a Lariat Capping Ribozyme to Study Cap Function In Vivo. in Ribozymes: Methods and Protocols. vol. 2167, Humana Press, Methods in Molecular Biology, vol. 2167, pp. 271-285. https://doi.org/10.1007/978-1-0716-0716-9_15

APA

Pietschmann, M., Tempel, G., Halladjian, M., Krogh, N., & Nielsen, H. (2021). Use of a Lariat Capping Ribozyme to Study Cap Function In Vivo. In Ribozymes: Methods and Protocols (Vol. 2167, pp. 271-285). Humana Press. Methods in Molecular Biology Vol. 2167 https://doi.org/10.1007/978-1-0716-0716-9_15

Vancouver

Pietschmann M, Tempel G, Halladjian M, Krogh N, Nielsen H. Use of a Lariat Capping Ribozyme to Study Cap Function In Vivo. In Ribozymes: Methods and Protocols. Vol. 2167. Humana Press. 2021. p. 271-285. (Methods in Molecular Biology, Vol. 2167). https://doi.org/10.1007/978-1-0716-0716-9_15

Author

Pietschmann, Max ; Tempel, Gregor ; Halladjian, Maral ; Krogh, Nicolai ; Nielsen, Henrik. / Use of a Lariat Capping Ribozyme to Study Cap Function In Vivo. Ribozymes: Methods and Protocols. Vol. 2167 Humana Press, 2021. pp. 271-285 (Methods in Molecular Biology, Vol. 2167).

Bibtex

@inbook{b7b8c8c14b0c4fa48740afc3f241d6b0,
title = "Use of a Lariat Capping Ribozyme to Study Cap Function In Vivo",
abstract = "A lariat cap is a naturally occurring substitute of a conventional mRNA cap and is found in a particular genomic setting in a few eukaryotic microorganisms. It is installed by the lariat capping ribozyme acting in cis. In principle, any RNA molecule in any organism can be equipped with a lariat cap in vivo when expressed downstream of a lariat capping ribozyme. Lariat capping is thus a versatile tool for studying the importance of the 5' end structure of RNA molecules. In this chapter, we present protocols to validate the presence of the lariat cap and measure the efficiency of in vivo cleavage by the lariat capping ribozyme.",
author = "Max Pietschmann and Gregor Tempel and Maral Halladjian and Nicolai Krogh and Henrik Nielsen",
year = "2021",
doi = "10.1007/978-1-0716-0716-9_15",
language = "English",
isbn = "978-1-0716-0715-2",
volume = "2167",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "271--285",
booktitle = "Ribozymes",
address = "United States",

}

RIS

TY - CHAP

T1 - Use of a Lariat Capping Ribozyme to Study Cap Function In Vivo

AU - Pietschmann, Max

AU - Tempel, Gregor

AU - Halladjian, Maral

AU - Krogh, Nicolai

AU - Nielsen, Henrik

PY - 2021

Y1 - 2021

N2 - A lariat cap is a naturally occurring substitute of a conventional mRNA cap and is found in a particular genomic setting in a few eukaryotic microorganisms. It is installed by the lariat capping ribozyme acting in cis. In principle, any RNA molecule in any organism can be equipped with a lariat cap in vivo when expressed downstream of a lariat capping ribozyme. Lariat capping is thus a versatile tool for studying the importance of the 5' end structure of RNA molecules. In this chapter, we present protocols to validate the presence of the lariat cap and measure the efficiency of in vivo cleavage by the lariat capping ribozyme.

AB - A lariat cap is a naturally occurring substitute of a conventional mRNA cap and is found in a particular genomic setting in a few eukaryotic microorganisms. It is installed by the lariat capping ribozyme acting in cis. In principle, any RNA molecule in any organism can be equipped with a lariat cap in vivo when expressed downstream of a lariat capping ribozyme. Lariat capping is thus a versatile tool for studying the importance of the 5' end structure of RNA molecules. In this chapter, we present protocols to validate the presence of the lariat cap and measure the efficiency of in vivo cleavage by the lariat capping ribozyme.

U2 - 10.1007/978-1-0716-0716-9_15

DO - 10.1007/978-1-0716-0716-9_15

M3 - Book chapter

C2 - 32712925

SN - 978-1-0716-0715-2

VL - 2167

T3 - Methods in Molecular Biology

SP - 271

EP - 285

BT - Ribozymes

PB - Humana Press

ER -

ID: 252108511