The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2'-O-Me sites

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Standard

The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2'-O-Me sites. / Ramachandran, Sowmya; Krogh, Nicolai; Jørgensen, Tor Erik; Johansen, Steinar Daae; Nielsen, Henrik; Babiak, Igor.

In: R N A, Vol. 26, No. 12, 12.2020, p. 1919-1934.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ramachandran, S, Krogh, N, Jørgensen, TE, Johansen, SD, Nielsen, H & Babiak, I 2020, 'The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2'-O-Me sites', R N A, vol. 26, no. 12, pp. 1919-1934. https://doi.org/10.1261/rna.076760.120

APA

Ramachandran, S., Krogh, N., Jørgensen, T. E., Johansen, S. D., Nielsen, H., & Babiak, I. (2020). The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2'-O-Me sites. R N A, 26(12), 1919-1934. https://doi.org/10.1261/rna.076760.120

Vancouver

Ramachandran S, Krogh N, Jørgensen TE, Johansen SD, Nielsen H, Babiak I. The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2'-O-Me sites. R N A. 2020 Dec;26(12):1919-1934. https://doi.org/10.1261/rna.076760.120

Author

Ramachandran, Sowmya ; Krogh, Nicolai ; Jørgensen, Tor Erik ; Johansen, Steinar Daae ; Nielsen, Henrik ; Babiak, Igor. / The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2'-O-Me sites. In: R N A. 2020 ; Vol. 26, No. 12. pp. 1919-1934.

Bibtex

@article{5c005dc9df0d4e85ab4b19796045ce7f,
title = "The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2'-O-Me sites",
abstract = "During zebrafish development, an early type of rRNA is gradually replaced by a late type that is substantially different in sequence. We applied RiboMeth-seq to rRNA from developmental stages for profiling of 2'-O-Me, to learn if changes in methylation pattern were a component of the shift. We compiled a catalog of 2'-O-Me sites and cognate box C/D guide RNAs comprising 98 high-confidence sites, including 10 sites that were not known from other vertebrates, one of which was specific to late-type rRNA. We identified a subset of sites that changed in methylation status during development and found that some of these could be explained by availability of their cognate SNORDs. Sites that changed during development were enriched in the novel sites revealed in zebrafish. We propose that the early type of rRNA is a specialized form and that its structure and ribose methylation pattern may be an adaptation to features of development, including translation of specific maternal mRNAs.",
author = "Sowmya Ramachandran and Nicolai Krogh and J{\o}rgensen, {Tor Erik} and Johansen, {Steinar Daae} and Henrik Nielsen and Igor Babiak",
note = "{\textcopyright} 2020 Ramachandran et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.",
year = "2020",
month = dec,
doi = "10.1261/rna.076760.120",
language = "English",
volume = "26",
pages = "1919--1934",
journal = "RNA",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
number = "12",

}

RIS

TY - JOUR

T1 - The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2'-O-Me sites

AU - Ramachandran, Sowmya

AU - Krogh, Nicolai

AU - Jørgensen, Tor Erik

AU - Johansen, Steinar Daae

AU - Nielsen, Henrik

AU - Babiak, Igor

N1 - © 2020 Ramachandran et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

PY - 2020/12

Y1 - 2020/12

N2 - During zebrafish development, an early type of rRNA is gradually replaced by a late type that is substantially different in sequence. We applied RiboMeth-seq to rRNA from developmental stages for profiling of 2'-O-Me, to learn if changes in methylation pattern were a component of the shift. We compiled a catalog of 2'-O-Me sites and cognate box C/D guide RNAs comprising 98 high-confidence sites, including 10 sites that were not known from other vertebrates, one of which was specific to late-type rRNA. We identified a subset of sites that changed in methylation status during development and found that some of these could be explained by availability of their cognate SNORDs. Sites that changed during development were enriched in the novel sites revealed in zebrafish. We propose that the early type of rRNA is a specialized form and that its structure and ribose methylation pattern may be an adaptation to features of development, including translation of specific maternal mRNAs.

AB - During zebrafish development, an early type of rRNA is gradually replaced by a late type that is substantially different in sequence. We applied RiboMeth-seq to rRNA from developmental stages for profiling of 2'-O-Me, to learn if changes in methylation pattern were a component of the shift. We compiled a catalog of 2'-O-Me sites and cognate box C/D guide RNAs comprising 98 high-confidence sites, including 10 sites that were not known from other vertebrates, one of which was specific to late-type rRNA. We identified a subset of sites that changed in methylation status during development and found that some of these could be explained by availability of their cognate SNORDs. Sites that changed during development were enriched in the novel sites revealed in zebrafish. We propose that the early type of rRNA is a specialized form and that its structure and ribose methylation pattern may be an adaptation to features of development, including translation of specific maternal mRNAs.

U2 - 10.1261/rna.076760.120

DO - 10.1261/rna.076760.120

M3 - Journal article

C2 - 32912962

VL - 26

SP - 1919

EP - 1934

JO - RNA

JF - RNA

SN - 1355-8382

IS - 12

ER -

ID: 251944900