T-cell dysfunction in HIV-1-infected patients with impaired recovery of CD4 cells despite suppression of viral replication
Research output: Contribution to journal › Journal article › Research › peer-review
INTRODUCTION: CD4 T-cell recovery is impeded in some HIVinfected patients despite successful combination antiretroviral therapy (cART) with suppressed HIV RNA. We hypothesized that T-cell dysfunction would be increased in these patients. METHODS: In the Danish HIV Cohort Study, we identified HIV-1-infected patients initiating cART with a CD4 cell count <100 cells per microliter, followed by HIV RNA <50 copies per milliliter for 3 years. Patients with a CD4 count <200 cells per microliter after 3 years were identified as cases; 42 patients with a CD4 count > or = 200 cells per microliter were selected as controls. Six-color flow cytometry was performed on whole blood. Cytokine levels in supernatants from whole blood stimulations were assessed. RESULTS: The case and control groups comprised 18 and 35 patients, respectively. Cases were older than controls (median: 54/46 years). The fraction of CD28+ cells was decreased among cases in the CD4+ and CD8+ T-cell subsets (P = 0.0014/P = 0.0349) and in the corresponding naive subsets (P = 0.0011/P , 0.0001). Cases had higher expression of human leukocyte antigen (HLA)-DR on naive CD4 and CD8 T cells (P = 0.0007/P = 0.0028). The production of interleukin (IL)-10 and IL-2 to phytohemagglutinin was decreased in cases (P < 0.0001/P = 0.019). CONCLUSIONS: Patients with impaired CD4 recovery shared a dysregulated T-cell phenotype with low CD28, high HLA-DR expression, and low IL-2 and IL-10 production.
Original language | English |
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Journal | J A I D S |
Volume | 53 |
Issue number | 3 |
Pages (from-to) | 303-10 |
Number of pages | 8 |
ISSN | 1525-4135 |
DOIs | |
Publication status | Published - 1 Mar 2010 |
Bibliographical note
Keywords: Adult; Anti-HIV Agents; Antigens, CD28; CD4 Lymphocyte Count; CD4-Positive T-Lymphocytes; Denmark; Female; Flow Cytometry; HIV Infections; HLA-DR Antigens; Humans; Interleukin-10; Interleukin-2; Male; Middle Aged; Viral Load
ID: 21458704