Synergy and oxygen adaptation for development of next-generation probiotics
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Synergy and oxygen adaptation for development of next-generation probiotics. / Khan, Muhammad Tanweer; Dwibedi, Chinmay; Sundh, Daniel; Pradhan, Meenakshi; Kraft, Jamie D.; Caesar, Robert; Tremaroli, Valentina; Lorentzon, Mattias; Bäckhed, Fredrik.
In: Nature, Vol. 620, 2023, p. 381-385.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Synergy and oxygen adaptation for development of next-generation probiotics
AU - Khan, Muhammad Tanweer
AU - Dwibedi, Chinmay
AU - Sundh, Daniel
AU - Pradhan, Meenakshi
AU - Kraft, Jamie D.
AU - Caesar, Robert
AU - Tremaroli, Valentina
AU - Lorentzon, Mattias
AU - Bäckhed, Fredrik
N1 - Publisher Copyright: © 2023, The Author(s).
PY - 2023
Y1 - 2023
N2 - The human gut microbiota has gained interest as an environmental factor that may contribute to health or disease 1. The development of next-generation probiotics is a promising strategy to modulate the gut microbiota and improve human health; however, several key candidate next-generation probiotics are strictly anaerobic 2 and may require synergy with other bacteria for optimal growth. Faecalibacterium prausnitzii is a highly prevalent and abundant human gut bacterium associated with human health, but it has not yet been developed into probiotic formulations 2. Here we describe the co-isolation of F. prausnitzii and Desulfovibrio piger, a sulfate-reducing bacterium, and their cross-feeding for growth and butyrate production. To produce a next-generation probiotic formulation, we adapted F. prausnitzii to tolerate oxygen exposure, and, in proof-of-concept studies, we demonstrate that the symbiotic product is tolerated by mice and humans (ClinicalTrials.gov identifier: NCT03728868) and is detected in the human gut in a subset of study participants. Our study describes a technology for the production of next-generation probiotics based on the adaptation of strictly anaerobic bacteria to tolerate oxygen exposures without a reduction in potential beneficial properties. Our technology may be used for the development of other strictly anaerobic strains as next-generation probiotics.
AB - The human gut microbiota has gained interest as an environmental factor that may contribute to health or disease 1. The development of next-generation probiotics is a promising strategy to modulate the gut microbiota and improve human health; however, several key candidate next-generation probiotics are strictly anaerobic 2 and may require synergy with other bacteria for optimal growth. Faecalibacterium prausnitzii is a highly prevalent and abundant human gut bacterium associated with human health, but it has not yet been developed into probiotic formulations 2. Here we describe the co-isolation of F. prausnitzii and Desulfovibrio piger, a sulfate-reducing bacterium, and their cross-feeding for growth and butyrate production. To produce a next-generation probiotic formulation, we adapted F. prausnitzii to tolerate oxygen exposure, and, in proof-of-concept studies, we demonstrate that the symbiotic product is tolerated by mice and humans (ClinicalTrials.gov identifier: NCT03728868) and is detected in the human gut in a subset of study participants. Our study describes a technology for the production of next-generation probiotics based on the adaptation of strictly anaerobic bacteria to tolerate oxygen exposures without a reduction in potential beneficial properties. Our technology may be used for the development of other strictly anaerobic strains as next-generation probiotics.
U2 - 10.1038/s41586-023-06378-w
DO - 10.1038/s41586-023-06378-w
M3 - Journal article
C2 - 37532933
AN - SCOPUS:85166553228
VL - 620
SP - 381
EP - 385
JO - Nature Genetics
JF - Nature Genetics
SN - 1061-4036
ER -
ID: 362320465