Structural features of five types of maize starch granule subgroups sorted by flow cytometry
Research output: Contribution to journal › Journal article › Research › peer-review
Subgroups of starch granules from five maize phenotypes including waxy-, normal-, popcorn-, sweet corn- and high-amylose maize were sorted by flow cytometry (FC) utilizing the side scatter channel (SSC) and forward scatter channel (FSC). SSC and FSC mainly reflecting internal object complexity, and object size, respectively. Subgroups with higher FSC signal always showed higher SSC signal, indicating larger granules exhibited higher internal structural complexity. Wide-angle and small-angle X-ray scattering analysis showed that the subgroups showing high SSC signal intensity also had high lamellar scattering intensity, and low crystallinity. Vibrational transitions of bonds analyzed by Fourier Transform Infrared Spectroscopy (FT-IR) showed that the subgroups of maize starches, except sweet corn starch, with high SSC signal had high intensities at 1045 and 1022 cm−1. Hence, our data demonstrate that the structural complexity detected by the SSC signal is mainly associated with lamellar and crystalline features of starch granules.
| Original language | English |
|---|---|
| Article number | 129657 |
| Journal | Food Chemistry |
| Volume | 356 |
| Number of pages | 9 |
| ISSN | 0308-8146 |
| DOIs | |
| Publication status | Published - 15 Sep 2021 |
Bibliographical note
Funding Information:
This work was financially supported by Germplasm Protection and Utilization in Shaanxi Province in China (Grant No. 20171010000004 ). Mr. Zhong also would like to thank the China Scholarship Council (CSC) funding for his PhD studies at University of Copenhagen (UCPH), Denmark.
Publisher Copyright:
© 2021 Elsevier Ltd
- Crystalline structure, Flow cytometry, Granular complexity, Lamellar structure, Maize starch
Research areas
ID: 306966188