Sequence of genome segment 9 of bluetongue virus (serotype 1, South Africa) and expression analysis demonstrating that different forms of VP6 are derived from initiation of protein synthesis at two distinct sites
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Sequence of genome segment 9 of bluetongue virus (serotype 1, South Africa) and expression analysis demonstrating that different forms of VP6 are derived from initiation of protein synthesis at two distinct sites. / Wade-Evans, A. M.; Mertens, P. P.C.; Belsham, G. J.
In: Journal of General Virology, Vol. 73, No. 11, 1992, p. 3023-3026.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Sequence of genome segment 9 of bluetongue virus (serotype 1, South Africa) and expression analysis demonstrating that different forms of VP6 are derived from initiation of protein synthesis at two distinct sites
AU - Wade-Evans, A. M.
AU - Mertens, P. P.C.
AU - Belsham, G. J.
PY - 1992
Y1 - 1992
N2 - Bluetongue virus (BTV) VP6 is often resolved into two closely migrating bands by SDS-PAGE (VP6 and VP6a. RNA segment 9 of BTV-serotype 1 South Africa (encoding VP6) has been cloned as cDNA, and the complete sequence has been determined. Expression of this clone both in vitro and in tissue culture produced the same polypeptide doublet as seen previously in extracts from BTV-infected cells. Modification of the cDNA, including the removal of the first initiation codon, demonstrated that the two forms of VP6 are derived from initiation of protein synthesis at two distinct sites and not by post-translational modification.
AB - Bluetongue virus (BTV) VP6 is often resolved into two closely migrating bands by SDS-PAGE (VP6 and VP6a. RNA segment 9 of BTV-serotype 1 South Africa (encoding VP6) has been cloned as cDNA, and the complete sequence has been determined. Expression of this clone both in vitro and in tissue culture produced the same polypeptide doublet as seen previously in extracts from BTV-infected cells. Modification of the cDNA, including the removal of the first initiation codon, demonstrated that the two forms of VP6 are derived from initiation of protein synthesis at two distinct sites and not by post-translational modification.
UR - http://www.scopus.com/inward/record.url?scp=0026474982&partnerID=8YFLogxK
U2 - 10.1099/0022-1317-73-11-3023
DO - 10.1099/0022-1317-73-11-3023
M3 - Journal article
C2 - 1331303
AN - SCOPUS:0026474982
VL - 73
SP - 3023
EP - 3026
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 11
ER -
ID: 381222971