Proteomics identifies differences in fibrotic potential of extracellular vesicles from human tendon and muscle fibroblasts

Research output: Contribution to journal › Journal article › Research › peer-review

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Proteomics identifies differences in fibrotic potential of extracellular vesicles from human tendon and muscle fibroblasts. / Yeung, Ching-yan Chloé; Schoof, Erwin M.; Tamáš, Michal; Mackey, Abigail L.; Kjaer, Michael.

In: Cell Communication and Signaling, Vol. 18, No. 1, 177, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Yeung, CC, Schoof, EM, Tamáš, M, Mackey, AL & Kjaer, M 2020, 'Proteomics identifies differences in fibrotic potential of extracellular vesicles from human tendon and muscle fibroblasts', Cell Communication and Signaling, vol. 18, no. 1, 177. https://doi.org/10.1186/s12964-020-00669-9

APA

Yeung, C. C., Schoof, E. M., Tamáš, M., Mackey, A. L., & Kjaer, M. (2020). Proteomics identifies differences in fibrotic potential of extracellular vesicles from human tendon and muscle fibroblasts. Cell Communication and Signaling, 18(1), [177]. https://doi.org/10.1186/s12964-020-00669-9

Vancouver

Yeung CC, Schoof EM, Tamáš M, Mackey AL, Kjaer M. Proteomics identifies differences in fibrotic potential of extracellular vesicles from human tendon and muscle fibroblasts. Cell Communication and Signaling. 2020;18(1). 177. https://doi.org/10.1186/s12964-020-00669-9

Author

Yeung, Ching-yan Chloé ; Schoof, Erwin M. ; Tamáš, Michal ; Mackey, Abigail L. ; Kjaer, Michael. / Proteomics identifies differences in fibrotic potential of extracellular vesicles from human tendon and muscle fibroblasts. In: Cell Communication and Signaling. 2020 ; Vol. 18, No. 1.

Bibtex

@article{ecfe09fbb3fb4ef995decf6f99b878c1,
title = "Proteomics identifies differences in fibrotic potential of extracellular vesicles from human tendon and muscle fibroblasts",
abstract = "BackgroundFibroblasts are the powerhouses responsible for the production and assembly of extracellular matrix (ECM). Their activity needs to be tightly controlled especially within the musculoskeletal system, where changes to ECM composition affect force transmission and mechanical loading that are required for effective movement of the body. Extracellular vesicles (EVs) are a mode of cell-cell communication within and between tissues, which has been largely characterised in cancer. However, it is unclear what the role of healthy fibroblast-derived EVs is during tissue homeostasis.MethodsHere, we performed proteomic analysis of small EVs derived from primary human muscle and tendon cells to identify the potential functions of healthy fibroblast-derived EVs.ResultsMass spectrometry-based proteomics revealed comprehensive profiles for small EVs released from healthy human fibroblasts from different tissues. We found that fibroblast-derived EVs were more similar than EVs from differentiating myoblasts, but there were significant differences between tendon fibroblast and muscle fibroblast EVs. Small EVs from tendon fibroblasts contained higher levels of proteins that support ECM synthesis, including TGFβ1, and muscle fibroblast EVs contained proteins that support myofiber function and components of the skeletal muscle matrix.ConclusionsOur data demonstrates a marked heterogeneity among healthy fibroblast-derived EVs, indicating shared tasks between EVs of skeletal muscle myoblasts and fibroblasts, whereas tendon fibroblast EVs could play a fibrotic role in human tendon tissue. These findings suggest an important role for EVs in tissue homeostasis of both tendon and skeletal muscle in humans.",
author = "Yeung, {Ching-yan Chlo{\'e}} and Schoof, {Erwin M.} and Michal Tam{\'a}{\v s} and Mackey, {Abigail L.} and Michael Kjaer",
year = "2020",
doi = "10.1186/s12964-020-00669-9",
language = "English",
volume = "18",
journal = "Cell Communication and Signaling",
issn = "1478-811X",
publisher = "BioMed Central Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - Proteomics identifies differences in fibrotic potential of extracellular vesicles from human tendon and muscle fibroblasts

AU - Yeung, Ching-yan Chloé

AU - Schoof, Erwin M.

AU - Tamáš, Michal

AU - Mackey, Abigail L.

AU - Kjaer, Michael

PY - 2020

Y1 - 2020

N2 - BackgroundFibroblasts are the powerhouses responsible for the production and assembly of extracellular matrix (ECM). Their activity needs to be tightly controlled especially within the musculoskeletal system, where changes to ECM composition affect force transmission and mechanical loading that are required for effective movement of the body. Extracellular vesicles (EVs) are a mode of cell-cell communication within and between tissues, which has been largely characterised in cancer. However, it is unclear what the role of healthy fibroblast-derived EVs is during tissue homeostasis.MethodsHere, we performed proteomic analysis of small EVs derived from primary human muscle and tendon cells to identify the potential functions of healthy fibroblast-derived EVs.ResultsMass spectrometry-based proteomics revealed comprehensive profiles for small EVs released from healthy human fibroblasts from different tissues. We found that fibroblast-derived EVs were more similar than EVs from differentiating myoblasts, but there were significant differences between tendon fibroblast and muscle fibroblast EVs. Small EVs from tendon fibroblasts contained higher levels of proteins that support ECM synthesis, including TGFβ1, and muscle fibroblast EVs contained proteins that support myofiber function and components of the skeletal muscle matrix.ConclusionsOur data demonstrates a marked heterogeneity among healthy fibroblast-derived EVs, indicating shared tasks between EVs of skeletal muscle myoblasts and fibroblasts, whereas tendon fibroblast EVs could play a fibrotic role in human tendon tissue. These findings suggest an important role for EVs in tissue homeostasis of both tendon and skeletal muscle in humans.

AB - BackgroundFibroblasts are the powerhouses responsible for the production and assembly of extracellular matrix (ECM). Their activity needs to be tightly controlled especially within the musculoskeletal system, where changes to ECM composition affect force transmission and mechanical loading that are required for effective movement of the body. Extracellular vesicles (EVs) are a mode of cell-cell communication within and between tissues, which has been largely characterised in cancer. However, it is unclear what the role of healthy fibroblast-derived EVs is during tissue homeostasis.MethodsHere, we performed proteomic analysis of small EVs derived from primary human muscle and tendon cells to identify the potential functions of healthy fibroblast-derived EVs.ResultsMass spectrometry-based proteomics revealed comprehensive profiles for small EVs released from healthy human fibroblasts from different tissues. We found that fibroblast-derived EVs were more similar than EVs from differentiating myoblasts, but there were significant differences between tendon fibroblast and muscle fibroblast EVs. Small EVs from tendon fibroblasts contained higher levels of proteins that support ECM synthesis, including TGFβ1, and muscle fibroblast EVs contained proteins that support myofiber function and components of the skeletal muscle matrix.ConclusionsOur data demonstrates a marked heterogeneity among healthy fibroblast-derived EVs, indicating shared tasks between EVs of skeletal muscle myoblasts and fibroblasts, whereas tendon fibroblast EVs could play a fibrotic role in human tendon tissue. These findings suggest an important role for EVs in tissue homeostasis of both tendon and skeletal muscle in humans.

U2 - 10.1186/s12964-020-00669-9

DO - 10.1186/s12964-020-00669-9

M3 - Journal article

C2 - 33148271

VL - 18

JO - Cell Communication and Signaling

JF - Cell Communication and Signaling

SN - 1478-811X

IS - 1

M1 - 177

ER -

ID: 250916912