Phylogenetic characterisation of the GL sequences of equine arteritis virus isolated from semen of asymptomatic stallions and fatal cases of equine viral arteritis in Denmark
Research output: Contribution to journal › Journal article › Research › peer-review
The study describes for the first time the phylogenetic relationship between equine arteritis virus (EAV) isolated from asymptomatic virus-shedding stallions and fatal cases of equine viral arteritis (EVA) in an European country. EAV was isolated from three dead foals and an aborted foetus during three different outbreaks of EVA. From these fatalities, the complete open reading frame 5, encoding the EAV GL protein, was amplified by reverse transcription-polymerase chain reaction and subjected to nucleotide sequence analysis. Furthermore, DNA sequences were obtained from virus isolated from semen samples of seven virus-shedding, but clinically healthy, Danish stallions. DNA sequence alignment revealed an overall divergence of 0-14 and 0-10% at the nucleotide and amino acid levels, respectively. Phylogenetic analysis including 24 previously published sequences revealed that European as well as North American "types" of EAV were present in the semen of asymptomatic carrier stallions and in fatal cases of EVA. Our results reveal that the presence of EAV-shedding stallions in Denmark represents a potential source of severe EVA.
Original language | English |
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Journal | Veterinary Microbiology |
Volume | 80 |
Issue number | 4 |
Pages (from-to) | 339-346 |
Number of pages | 8 |
ISSN | 0378-1135 |
DOIs | |
Publication status | Published - 22 Jun 2001 |
Externally published | Yes |
- Epidemiology, Equine arteritis virus, G protein, Horse, Open reading frame 5, Phylogeny
Research areas
ID: 247400769