Oligonucleotide fishing for STAT6: cross-talk between IL-4 and chemokines.
Research output: Contribution to journal › Journal article › Research › peer-review
Signal transducer and activator of transcription 6 (STAT6) is essential for the biological activities of interleukin-4 (IL-4) and the development of allergic responses in mice. Here we report on a sensitive and specific assay for STAT6 activation in response to IL-4. We took advantage of double-stranded oligonucleotide probes containing a STAT6-binding gene-sequence from the promotor of the immunoglobulin heavy chain germline epsilon transcript to study the IL-4-induced DNA binding of STAT6. Using these probes, we show that repeated adjacent STAT6-binding sites result in enhanced STAT6-DNA binding. Moreover, the distance between the binding sites is critical for STAT-DNA binding, i.e. STAT6 binding is decreased at distances above 20 nucleotides between neighbouring binding sites. Using this assay to study cross-talk between IL-4 and chemokines, we provide evidence that MIP-1beta and MIG inhibit IL-4-induced STAT6 activation, whereas other chemokines and cytokines do not. In conclusion, our data show that oligonucleotide fishing is a supplementary tool for studying cytokine cross-talk at a genomic level.
Original language | English |
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Journal | Experimental and Clinical Immunogenetics |
Volume | 18 |
Issue number | 4 |
Pages (from-to) | 233-41 |
Number of pages | 8 |
ISSN | 0254-9670 |
Publication status | Published - 2001 |
Bibliographical note
Keywords: Animals; Base Sequence; Binding Sites; CD4-Positive T-Lymphocytes; Cell Line; Chemokines; Cytokines; DNA; Humans; Interleukin-4; Mice; Molecular Sequence Data; Oligonucleotide Probes; Phosphorylation; STAT6 Transcription Factor; Trans-Activators; Tyrosine
ID: 8746199