Methods for demonstration of enzyme activity in muscle fibres at the muscle/bone interface in demineralized tissue

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Standard

Methods for demonstration of enzyme activity in muscle fibres at the muscle/bone interface in demineralized tissue. / Kirkeby, S; Vilmann, H.

In: Histochemistry, Vol. 70, No. 2, 1981, p. 115-21.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kirkeby, S & Vilmann, H 1981, 'Methods for demonstration of enzyme activity in muscle fibres at the muscle/bone interface in demineralized tissue', Histochemistry, vol. 70, no. 2, pp. 115-21.

APA

Kirkeby, S., & Vilmann, H. (1981). Methods for demonstration of enzyme activity in muscle fibres at the muscle/bone interface in demineralized tissue. Histochemistry, 70(2), 115-21.

Vancouver

Kirkeby S, Vilmann H. Methods for demonstration of enzyme activity in muscle fibres at the muscle/bone interface in demineralized tissue. Histochemistry. 1981;70(2):115-21.

Author

Kirkeby, S ; Vilmann, H. / Methods for demonstration of enzyme activity in muscle fibres at the muscle/bone interface in demineralized tissue. In: Histochemistry. 1981 ; Vol. 70, No. 2. pp. 115-21.

Bibtex

@article{c63d7f00f69211ddbf70000ea68e967b,
title = "Methods for demonstration of enzyme activity in muscle fibres at the muscle/bone interface in demineralized tissue",
abstract = "A method for demonstration of activity for ATPase and various oxidative enzymes (succinic dehydrogenase, alpha-glycerophosphate dehydrogenase, and lactic dehydrogenase) in muscle/bone sections of fixed and demineralized tissue has been developed. It was found that it is possible to preserve considerable amounts of the above mentioned enzymes in the muscle fibres at the muscle/bone interfaces. The best results were obtained after 20 min fixation, and 2-3 weeks of storage in MgNa2EDTA containing media. As the same technique previously has been used to describe patterns of resorption and deposition with the aid of a mapping of presence of phosphomonoesterases on bone surfaces, the method may be used to study possible biochemical interactions between bone and muscle tissue at the muscle/bone interface.",
author = "S Kirkeby and H Vilmann",
note = "Keywords: Adenosine Triphosphatases; Animals; Bone and Bones; Buffers; Glycerolphosphate Dehydrogenase; Histocytochemistry; L-Lactate Dehydrogenase; Methods; Muscles; Rats; Succinate Dehydrogenase",
year = "1981",
language = "English",
volume = "70",
pages = "115--21",
journal = "Histochemistry",
issn = "0301-5564",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Methods for demonstration of enzyme activity in muscle fibres at the muscle/bone interface in demineralized tissue

AU - Kirkeby, S

AU - Vilmann, H

N1 - Keywords: Adenosine Triphosphatases; Animals; Bone and Bones; Buffers; Glycerolphosphate Dehydrogenase; Histocytochemistry; L-Lactate Dehydrogenase; Methods; Muscles; Rats; Succinate Dehydrogenase

PY - 1981

Y1 - 1981

N2 - A method for demonstration of activity for ATPase and various oxidative enzymes (succinic dehydrogenase, alpha-glycerophosphate dehydrogenase, and lactic dehydrogenase) in muscle/bone sections of fixed and demineralized tissue has been developed. It was found that it is possible to preserve considerable amounts of the above mentioned enzymes in the muscle fibres at the muscle/bone interfaces. The best results were obtained after 20 min fixation, and 2-3 weeks of storage in MgNa2EDTA containing media. As the same technique previously has been used to describe patterns of resorption and deposition with the aid of a mapping of presence of phosphomonoesterases on bone surfaces, the method may be used to study possible biochemical interactions between bone and muscle tissue at the muscle/bone interface.

AB - A method for demonstration of activity for ATPase and various oxidative enzymes (succinic dehydrogenase, alpha-glycerophosphate dehydrogenase, and lactic dehydrogenase) in muscle/bone sections of fixed and demineralized tissue has been developed. It was found that it is possible to preserve considerable amounts of the above mentioned enzymes in the muscle fibres at the muscle/bone interfaces. The best results were obtained after 20 min fixation, and 2-3 weeks of storage in MgNa2EDTA containing media. As the same technique previously has been used to describe patterns of resorption and deposition with the aid of a mapping of presence of phosphomonoesterases on bone surfaces, the method may be used to study possible biochemical interactions between bone and muscle tissue at the muscle/bone interface.

M3 - Journal article

C2 - 6452430

VL - 70

SP - 115

EP - 121

JO - Histochemistry

JF - Histochemistry

SN - 0301-5564

IS - 2

ER -

ID: 10209954