Interaction between prostaglandins of the E-type with a urinary component from halothane anesthetized rats.
Research output: Contribution to journal › Journal article › Research › peer-review
Prostaglandins of the E-type (PGE's) were found to react or combine with a urinary metabolite of Halothane yielding products which were left unrecovered during the purification procedure preceding specific radioimmunoassay of PGE2. The products were retained on sephadex LH-20 columns, and showed on thin layer silica gel plates (TLC) Rf values lower than those of the parent PGE-compounds. The product formation is supposed to involve the beta-hydroxyketone system of PGE, since PG's of the F and A type were unaffected. The product formation could be avoided by inducing anaesthesia with Hexobarbitone and maintaining the anaesthesia with Halothane-nitrous oxide or it could be reversed by adding barbiturates to urine samples obtained from animals anaesthetized with Halothane-nitrous oxide alone. The barbiturates effectively competed with PGE for the metabolite leaving PGE to behave normally on sephadex LH-20 and TLC, thus enabling us to evaluate correctly the PGE2 content by RIA.
Original language | English |
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Journal | Prostaglandins & Other Lipid Mediators |
Volume | 22 |
Issue number | 6 |
Pages (from-to) | 893-902 |
Number of pages | 9 |
ISSN | 1098-8823 |
Publication status | Published - 1981 |
Bibliographical note
Keywords: Anesthetics; Animals; Chromatography, Gel; Chromatography, Thin Layer; Dinoprost; Dinoprostone; Halothane; Hexobarbital; Male; Nitrous Oxide; Prostaglandins A; Prostaglandins E; Prostaglandins F; Radioimmunoassay; Rats; Rats, Inbred Strains
ID: 8440591