Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria
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Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria. / Mootha, Vamsi K; Bunkenborg, Jakob; Olsen, Jesper V; Hjerrild, Majbrit; Wisniewski, Jacek R; Stahl, Erich; Bolouri, Marjan S; Ray, Heta N; Sihag, Smita; Kamal, Michael; Patterson, Nick; Lander, Eric S; Mann, Matthias.
In: Cell, Vol. 115, No. 5, 2003, p. 629-40.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria
AU - Mootha, Vamsi K
AU - Bunkenborg, Jakob
AU - Olsen, Jesper V
AU - Hjerrild, Majbrit
AU - Wisniewski, Jacek R
AU - Stahl, Erich
AU - Bolouri, Marjan S
AU - Ray, Heta N
AU - Sihag, Smita
AU - Kamal, Michael
AU - Patterson, Nick
AU - Lander, Eric S
AU - Mann, Matthias
N1 - Keywords: Animals; Cell Line; Cell Respiration; DNA Fingerprinting; DNA, Mitochondrial; Gene Expression Regulation, Enzymologic; Humans; Male; Mice; Mitochondria; Mitochondrial Proteins; Oxidative Phosphorylation; Proteomics; RNA, Messenger
PY - 2003
Y1 - 2003
N2 - Mitochondria are tailored to meet the metabolic and signaling needs of each cell. To explore its molecular composition, we performed a proteomic survey of mitochondria from mouse brain, heart, kidney, and liver and combined the results with existing gene annotations to produce a list of 591 mitochondrial proteins, including 163 proteins not previously associated with this organelle. The protein expression data were largely concordant with large-scale surveys of RNA abundance and both measures indicate tissue-specific differences in organelle composition. RNA expression profiles across tissues revealed networks of mitochondrial genes that share functional and regulatory mechanisms. We also determined a larger "neighborhood" of genes whose expression is closely correlated to the mitochondrial genes. The combined analysis identifies specific genes of biological interest, such as candidates for mtDNA repair enzymes, offers new insights into the biogenesis and ancestry of mammalian mitochondria, and provides a framework for understanding the organelle's contribution to human disease.
AB - Mitochondria are tailored to meet the metabolic and signaling needs of each cell. To explore its molecular composition, we performed a proteomic survey of mitochondria from mouse brain, heart, kidney, and liver and combined the results with existing gene annotations to produce a list of 591 mitochondrial proteins, including 163 proteins not previously associated with this organelle. The protein expression data were largely concordant with large-scale surveys of RNA abundance and both measures indicate tissue-specific differences in organelle composition. RNA expression profiles across tissues revealed networks of mitochondrial genes that share functional and regulatory mechanisms. We also determined a larger "neighborhood" of genes whose expression is closely correlated to the mitochondrial genes. The combined analysis identifies specific genes of biological interest, such as candidates for mtDNA repair enzymes, offers new insights into the biogenesis and ancestry of mammalian mitochondria, and provides a framework for understanding the organelle's contribution to human disease.
M3 - Journal article
C2 - 14651853
VL - 115
SP - 629
EP - 640
JO - Cell
JF - Cell
SN - 0092-8674
IS - 5
ER -
ID: 16275847