In vivo calcium imaging shows that satellite glial cells have increased activity in painful states
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In vivo calcium imaging shows that satellite glial cells have increased activity in painful states. / Jager, Sara E.; Goodwin, George; Chisholm, Kim I.; Denk, Franziska.
In: Brain Communications, Vol. 6, No. 2, fcae013, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - In vivo calcium imaging shows that satellite glial cells have increased activity in painful states
AU - Jager, Sara E.
AU - Goodwin, George
AU - Chisholm, Kim I.
AU - Denk, Franziska
N1 - Publisher Copyright: © The Author(s) 2024.
PY - 2024
Y1 - 2024
N2 - Satellite glial cells are important for proper neuronal function of primary sensory neurons for which they provide homeostatic support. Most research on satellite glial cell function has been performed with in vitro studies, but recent advances in calcium imaging and transgenic mouse models have enabled this first in vivo study of single-cell satellite glial cell function in mouse models of inflammation and neuropathic pain. We found that in naïve conditions, satellite glial cells do not respond in a time-locked fashion to neuronal firing. In painful inflammatory and neuropathic states, we detected time-locked signals in a subset of satellite glial cells, but only with suprathreshold stimulation of the sciatic nerve. Surprisingly, therefore, we conclude that most calcium signals in satellite glial cells seem to develop at arbitrary intervals not directly linked to neuronal activity patterns. More in line with expectations, our experiments also revealed that the number of active satellite glial cells was increased under conditions of inflammation or nerve injury. This could reflect the increased requirement for homeostatic support across dorsal root ganglion neuron populations, which are more active during such painful states.
AB - Satellite glial cells are important for proper neuronal function of primary sensory neurons for which they provide homeostatic support. Most research on satellite glial cell function has been performed with in vitro studies, but recent advances in calcium imaging and transgenic mouse models have enabled this first in vivo study of single-cell satellite glial cell function in mouse models of inflammation and neuropathic pain. We found that in naïve conditions, satellite glial cells do not respond in a time-locked fashion to neuronal firing. In painful inflammatory and neuropathic states, we detected time-locked signals in a subset of satellite glial cells, but only with suprathreshold stimulation of the sciatic nerve. Surprisingly, therefore, we conclude that most calcium signals in satellite glial cells seem to develop at arbitrary intervals not directly linked to neuronal activity patterns. More in line with expectations, our experiments also revealed that the number of active satellite glial cells was increased under conditions of inflammation or nerve injury. This could reflect the increased requirement for homeostatic support across dorsal root ganglion neuron populations, which are more active during such painful states.
KW - dorsal root ganglion
KW - in vivo Ca imaging
KW - neuropathic pain
KW - peripheral nerve injury
KW - satellite glial cells
U2 - 10.1093/braincomms/fcae013
DO - 10.1093/braincomms/fcae013
M3 - Journal article
AN - SCOPUS:85187531770
VL - 6
JO - Brain Communications
JF - Brain Communications
SN - 2632-1297
IS - 2
M1 - fcae013
ER -
ID: 385898014