In situ monitoring of the formation of lipidic non-lamellar liquid crystalline depot formulations in synovial fluid

Research output: Contribution to journalJournal articleResearchpeer-review

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In situ monitoring of the formation of lipidic non-lamellar liquid crystalline depot formulations in synovial fluid. / Yaghmur, Anan; Rappolt, Michael; Jonassen, Anne Louise Uldall; Schmitt, Mechthild; Larsen, Susan Weng.

In: Journal of Colloid and Interface Science, Vol. 582, 15.01.2021, p. 773-781.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Yaghmur, A, Rappolt, M, Jonassen, ALU, Schmitt, M & Larsen, SW 2021, 'In situ monitoring of the formation of lipidic non-lamellar liquid crystalline depot formulations in synovial fluid', Journal of Colloid and Interface Science, vol. 582, pp. 773-781. https://doi.org/10.1016/j.jcis.2020.08.084

APA

Yaghmur, A., Rappolt, M., Jonassen, A. L. U., Schmitt, M., & Larsen, S. W. (2021). In situ monitoring of the formation of lipidic non-lamellar liquid crystalline depot formulations in synovial fluid. Journal of Colloid and Interface Science, 582, 773-781. https://doi.org/10.1016/j.jcis.2020.08.084

Vancouver

Yaghmur A, Rappolt M, Jonassen ALU, Schmitt M, Larsen SW. In situ monitoring of the formation of lipidic non-lamellar liquid crystalline depot formulations in synovial fluid. Journal of Colloid and Interface Science. 2021 Jan 15;582:773-781. https://doi.org/10.1016/j.jcis.2020.08.084

Author

Yaghmur, Anan ; Rappolt, Michael ; Jonassen, Anne Louise Uldall ; Schmitt, Mechthild ; Larsen, Susan Weng. / In situ monitoring of the formation of lipidic non-lamellar liquid crystalline depot formulations in synovial fluid. In: Journal of Colloid and Interface Science. 2021 ; Vol. 582. pp. 773-781.

Bibtex

@article{e4de578c006b4c96aa5608b65eb1f7f0,
title = "In situ monitoring of the formation of lipidic non-lamellar liquid crystalline depot formulations in synovial fluid",
abstract = "Administration of parenteral liquid crystalline phases, forming in-vivo with tunable nanostructural features and sustained release properties, offers an attractive approach for treatment of infections and local drug delivery. It has also a potential use for postoperative pain management after arthroscopic knee surgery. However, the optimal use of this drug delivery principle requires an improved understanding of the involved dynamic structural transitions after administration of low-viscous stimulus-responsive lipid precursors and their fate after direct contact with the biological environment. These precursors (preformulations) are typically based on a single biologically relevant lipid (or a lipid combination) with non-lamellar liquid crystalline phase forming propensity. In relation to liquid crystalline depot design for intra-articular drug delivery, it was our interest in the present study to shed light on such dynamic structural transitions by combining synchrotron SAXS with a remote controlled addition of synovial fluid (or buffer containing 2{\%} (w/v) albumin). This combination allowed for monitoring in real-time the hydration-triggered dynamic structural events on exposure of the lipid precursor (organic stock solution consisting of the binary lipid mixture of monoolein and castor oil) to excess synovial fluid (or excess buffer). The synchrotron SAXS findings indicate a fast generation of inverse bicontinuous cubic phases within few seconds. The effects of (i) the organic solvent N-methyl-2-pyrolidone (NMP), (ii) the lipid composition, and (iii) the albumin content on modulating the structures of the self-assembled lipid aggregates and the implications of the experimental findings in the design of liquid crystalline depots for intra-articular drug delivery are discussed.",
keywords = "Albumin, In situ forming drug delivery systems, Inverse bicontinuous cubic phase, Inverse hexagonal phase, Lyotropic liquid crystals, Small angle X-ray scattering, Stimulus-responsive precursors, Synchrotron light, Synovial fluid",
author = "Anan Yaghmur and Michael Rappolt and Jonassen, {Anne Louise Uldall} and Mechthild Schmitt and Larsen, {Susan Weng}",
year = "2021",
month = "1",
day = "15",
doi = "10.1016/j.jcis.2020.08.084",
language = "English",
volume = "582",
pages = "773--781",
journal = "Journal of Colloid and Interface Science",
issn = "0021-9797",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - In situ monitoring of the formation of lipidic non-lamellar liquid crystalline depot formulations in synovial fluid

AU - Yaghmur, Anan

AU - Rappolt, Michael

AU - Jonassen, Anne Louise Uldall

AU - Schmitt, Mechthild

AU - Larsen, Susan Weng

PY - 2021/1/15

Y1 - 2021/1/15

N2 - Administration of parenteral liquid crystalline phases, forming in-vivo with tunable nanostructural features and sustained release properties, offers an attractive approach for treatment of infections and local drug delivery. It has also a potential use for postoperative pain management after arthroscopic knee surgery. However, the optimal use of this drug delivery principle requires an improved understanding of the involved dynamic structural transitions after administration of low-viscous stimulus-responsive lipid precursors and their fate after direct contact with the biological environment. These precursors (preformulations) are typically based on a single biologically relevant lipid (or a lipid combination) with non-lamellar liquid crystalline phase forming propensity. In relation to liquid crystalline depot design for intra-articular drug delivery, it was our interest in the present study to shed light on such dynamic structural transitions by combining synchrotron SAXS with a remote controlled addition of synovial fluid (or buffer containing 2% (w/v) albumin). This combination allowed for monitoring in real-time the hydration-triggered dynamic structural events on exposure of the lipid precursor (organic stock solution consisting of the binary lipid mixture of monoolein and castor oil) to excess synovial fluid (or excess buffer). The synchrotron SAXS findings indicate a fast generation of inverse bicontinuous cubic phases within few seconds. The effects of (i) the organic solvent N-methyl-2-pyrolidone (NMP), (ii) the lipid composition, and (iii) the albumin content on modulating the structures of the self-assembled lipid aggregates and the implications of the experimental findings in the design of liquid crystalline depots for intra-articular drug delivery are discussed.

AB - Administration of parenteral liquid crystalline phases, forming in-vivo with tunable nanostructural features and sustained release properties, offers an attractive approach for treatment of infections and local drug delivery. It has also a potential use for postoperative pain management after arthroscopic knee surgery. However, the optimal use of this drug delivery principle requires an improved understanding of the involved dynamic structural transitions after administration of low-viscous stimulus-responsive lipid precursors and their fate after direct contact with the biological environment. These precursors (preformulations) are typically based on a single biologically relevant lipid (or a lipid combination) with non-lamellar liquid crystalline phase forming propensity. In relation to liquid crystalline depot design for intra-articular drug delivery, it was our interest in the present study to shed light on such dynamic structural transitions by combining synchrotron SAXS with a remote controlled addition of synovial fluid (or buffer containing 2% (w/v) albumin). This combination allowed for monitoring in real-time the hydration-triggered dynamic structural events on exposure of the lipid precursor (organic stock solution consisting of the binary lipid mixture of monoolein and castor oil) to excess synovial fluid (or excess buffer). The synchrotron SAXS findings indicate a fast generation of inverse bicontinuous cubic phases within few seconds. The effects of (i) the organic solvent N-methyl-2-pyrolidone (NMP), (ii) the lipid composition, and (iii) the albumin content on modulating the structures of the self-assembled lipid aggregates and the implications of the experimental findings in the design of liquid crystalline depots for intra-articular drug delivery are discussed.

KW - Albumin

KW - In situ forming drug delivery systems

KW - Inverse bicontinuous cubic phase

KW - Inverse hexagonal phase

KW - Lyotropic liquid crystals

KW - Small angle X-ray scattering

KW - Stimulus-responsive precursors

KW - Synchrotron light

KW - Synovial fluid

UR - http://www.scopus.com/inward/record.url?scp=85090271758&partnerID=8YFLogxK

U2 - 10.1016/j.jcis.2020.08.084

DO - 10.1016/j.jcis.2020.08.084

M3 - Journal article

AN - SCOPUS:85090271758

VL - 582

SP - 773

EP - 781

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

ER -

ID: 248499290