TY - CHAP

T1 - Gene Expression Analysis Using Agilent DNA Microarrays

AU - Stangegaard, Michael

N1 - ikke relevant

PY - 2009

Y1 - 2009

N2 - Hybridization of labeled cDNA to microarrays is an intuitively simple and a vastly underestimated process. If it is not performed, optimized, and standardized with the same attention to detail as e.g., RNA amplification, information may be overlooked or even lost. Careful balancing of the amount of labeled cDNA added to each slide reduces dye-bias and slide to slide variation. Efficient mixing of the hybridization solution throughout the hybridization reaction increases signals several fold. The amount of near perfect target-probe hybrids may be reduced by efficient stringency washes of the hybridized microarray slides.

AB - Hybridization of labeled cDNA to microarrays is an intuitively simple and a vastly underestimated process. If it is not performed, optimized, and standardized with the same attention to detail as e.g., RNA amplification, information may be overlooked or even lost. Careful balancing of the amount of labeled cDNA added to each slide reduces dye-bias and slide to slide variation. Efficient mixing of the hybridization solution throughout the hybridization reaction increases signals several fold. The amount of near perfect target-probe hybrids may be reduced by efficient stringency washes of the hybridized microarray slides.

KW - Faculty of Health and Medical Sciences

U2 - 10.1007/978-1-59745-538-1_9

DO - 10.1007/978-1-59745-538-1_9

M3 - Book chapter

C2 - 19381965

SN - 1064-3745

SN - 978-1-934115-69-5

VL - 529

T3 - Methods in Molecular Biology

SP - 133

EP - 145

BT - DNA Microarrays for Biomedical Research

A2 - Dufva, Hans Martin

PB - Springer Science+Business Media

CY - Spring Street, New York, NY 10013, USA

ER -