Feasibility of monitoring peripheral blood to detect emerging clones in children with acute lymphoblastic leukemia†

Research output: Contribution to journal › Journal article › Research › peer-review

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Feasibility of monitoring peripheral blood to detect emerging clones in children with acute lymphoblastic leukemia†. / Saliba, Jason; Evensen, Nikki A.; Meyer, Julia A.; Newman, Daniel; Nersting, Jacob; Narang, Sonali; Ma, Xiaotu; Schmiegelow, Kjeld; Carroll, William L.

In: Pediatric Blood & Cancer, Vol. 67, No. 7, e28306, 07.2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Saliba, J, Evensen, NA, Meyer, JA, Newman, D, Nersting, J, Narang, S, Ma, X, Schmiegelow, K & Carroll, WL 2020, 'Feasibility of monitoring peripheral blood to detect emerging clones in children with acute lymphoblastic leukemia†', Pediatric Blood & Cancer, vol. 67, no. 7, e28306. https://doi.org/10.1002/pbc.28306

APA

Saliba, J., Evensen, N. A., Meyer, J. A., Newman, D., Nersting, J., Narang, S., Ma, X., Schmiegelow, K., & Carroll, W. L. (2020). Feasibility of monitoring peripheral blood to detect emerging clones in children with acute lymphoblastic leukemia†. Pediatric Blood & Cancer, 67(7), [e28306]. https://doi.org/10.1002/pbc.28306

Vancouver

Saliba J, Evensen NA, Meyer JA, Newman D, Nersting J, Narang S et al. Feasibility of monitoring peripheral blood to detect emerging clones in children with acute lymphoblastic leukemia†. Pediatric Blood & Cancer. 2020 Jul;67(7). e28306. https://doi.org/10.1002/pbc.28306

Author

Saliba, Jason ; Evensen, Nikki A. ; Meyer, Julia A. ; Newman, Daniel ; Nersting, Jacob ; Narang, Sonali ; Ma, Xiaotu ; Schmiegelow, Kjeld ; Carroll, William L. / Feasibility of monitoring peripheral blood to detect emerging clones in children with acute lymphoblastic leukemia†. In: Pediatric Blood & Cancer. 2020 ; Vol. 67, No. 7.

Bibtex

@article{76e4625169264c26a44acab554e47b0a,
title = "Feasibility of monitoring peripheral blood to detect emerging clones in children with acute lymphoblastic leukemia†",
abstract = "Relapse-enriched somatic variants drive drug resistance in childhood acute lymphoblastic leukemia. We used digital droplet-based polymerase chain reaction to establish whether relapse-enriched mutations in emerging subclones could be detected in peripheral blood samples before frank relapse. Although limitations in sensitivity for some probes hindered detection of certain variants, we successfully detected variants in NT5C2 and PRPS1 at a fractional abundance of 0.005% to 0.3%, 41 to 116 days before relapse. As mutations in both these genes confer resistance to thiopurines, early detection protocols using peripheral blood could be implemented to preemptively alter maintenance therapy to extinguish resistant clones before overt relapse.",
keywords = "backtracking, clonal evolution, ddPCR, relapsed acute lymphoblastic leukemia, thiopurines",
author = "Jason Saliba and Evensen, {Nikki A.} and Meyer, {Julia A.} and Daniel Newman and Jacob Nersting and Sonali Narang and Xiaotu Ma and Kjeld Schmiegelow and Carroll, {William L.}",
year = "2020",
month = jul,
doi = "10.1002/pbc.28306",
language = "English",
volume = "67",
journal = "Pediatric Blood & Cancer",
issn = "1545-5009",
publisher = "JohnWiley & Sons, Inc.",
number = "7",

}

RIS

TY - JOUR

T1 - Feasibility of monitoring peripheral blood to detect emerging clones in children with acute lymphoblastic leukemia†

AU - Saliba, Jason

AU - Evensen, Nikki A.

AU - Meyer, Julia A.

AU - Newman, Daniel

AU - Nersting, Jacob

AU - Narang, Sonali

AU - Ma, Xiaotu

AU - Schmiegelow, Kjeld

AU - Carroll, William L.

PY - 2020/7

Y1 - 2020/7

N2 - Relapse-enriched somatic variants drive drug resistance in childhood acute lymphoblastic leukemia. We used digital droplet-based polymerase chain reaction to establish whether relapse-enriched mutations in emerging subclones could be detected in peripheral blood samples before frank relapse. Although limitations in sensitivity for some probes hindered detection of certain variants, we successfully detected variants in NT5C2 and PRPS1 at a fractional abundance of 0.005% to 0.3%, 41 to 116 days before relapse. As mutations in both these genes confer resistance to thiopurines, early detection protocols using peripheral blood could be implemented to preemptively alter maintenance therapy to extinguish resistant clones before overt relapse.

AB - Relapse-enriched somatic variants drive drug resistance in childhood acute lymphoblastic leukemia. We used digital droplet-based polymerase chain reaction to establish whether relapse-enriched mutations in emerging subclones could be detected in peripheral blood samples before frank relapse. Although limitations in sensitivity for some probes hindered detection of certain variants, we successfully detected variants in NT5C2 and PRPS1 at a fractional abundance of 0.005% to 0.3%, 41 to 116 days before relapse. As mutations in both these genes confer resistance to thiopurines, early detection protocols using peripheral blood could be implemented to preemptively alter maintenance therapy to extinguish resistant clones before overt relapse.

KW - backtracking

KW - clonal evolution

KW - ddPCR

KW - relapsed acute lymphoblastic leukemia

KW - thiopurines

U2 - 10.1002/pbc.28306

DO - 10.1002/pbc.28306

M3 - Journal article

C2 - 32391957

AN - SCOPUS:85084420194

VL - 67

JO - Pediatric Blood & Cancer

JF - Pediatric Blood & Cancer

SN - 1545-5009

IS - 7

M1 - e28306

ER -

ID: 244204495