Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor

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Standard

Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor. / Rao, C N; Castronovo, V; Schmitt, M C; Wewer, U M; Claysmith, A P; Liotta, L A; Sobel, M E.

In: Biochemistry, Vol. 28, No. 18, 05.09.1989, p. 7476-86.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rao, CN, Castronovo, V, Schmitt, MC, Wewer, UM, Claysmith, AP, Liotta, LA & Sobel, ME 1989, 'Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor', Biochemistry, vol. 28, no. 18, pp. 7476-86.

APA

Rao, C. N., Castronovo, V., Schmitt, M. C., Wewer, U. M., Claysmith, A. P., Liotta, L. A., & Sobel, M. E. (1989). Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor. Biochemistry, 28(18), 7476-86.

Vancouver

Rao CN, Castronovo V, Schmitt MC, Wewer UM, Claysmith AP, Liotta LA et al. Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor. Biochemistry. 1989 Sep 5;28(18):7476-86.

Author

Rao, C N ; Castronovo, V ; Schmitt, M C ; Wewer, U M ; Claysmith, A P ; Liotta, L A ; Sobel, M E. / Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor. In: Biochemistry. 1989 ; Vol. 28, No. 18. pp. 7476-86.

Bibtex

@article{09dd4e718b77469a880fcdf2ae683330,
title = "Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor",
abstract = "The high-affinity cellular receptor for the basement membrane component laminin is differentially expressed during tumor invasion and metastasis. A cDNA clone encoding the murine laminin receptor was isolated and identified on the basis of sequence homology to the human laminin receptor [Wewer et al. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 7137-7141]. Primer extension experiments demonstrated that the clone contained the complete 5' sequence of the murine laminin receptor mRNA. RNA blot data demonstrated a single-sized laminin receptor mRNA, approximately 1400 bases long, in human, mouse, and rat. The nascent laminin receptor predicted from the cDNA sequence is 295 amino acids long, with a molecular weight of 33,000, and contains one intradisulfide bridge, a short putative transmembrane domain, and an extracellular carboxy-terminal region which has abundant glutamic acid residues and multiple repeat sequences. The precursor of the laminin receptor is apparently smaller than the 67-kilodalton protein isolated from tissue. The apparent molecular weight on SDS-polyacrylamide gels of the rabbit reticulocyte cell-free translation product of selectively hybridized laminin receptor mRNA is 37,000. Antisera to three different domains of the cDNA-predicted receptor were used to study the relationship between the 37- and 67-kilodalton polypeptides. Antisera to cDNA-deduced synthetic peptides of the receptor immunoprecipitated a 37-kilodalton band both from cell-free translation products and from pulse-labeled cell extracts. On immunoblots of cell extracts, one antisynthetic peptide antiserum recognized only the 67-kilodalton receptor, while another antiserum identified both 37- and 67-kilodalton polypeptides, suggesting a precursor-product relationship between the two polypeptides.",
keywords = "Amino Acid Sequence, Animals, Base Sequence, DNA, Humans, Immunoblotting, Laminin, Mice, Molecular Sequence Data, Neoplasm Metastasis, Nucleic Acid Conformation, Nucleic Acid Hybridization, Protein Biosynthesis, Protein Conformation, Protein Precursors, RNA, Messenger, Rats, Receptors, Immunologic, Receptors, Laminin, Restriction Mapping, Sequence Homology, Nucleic Acid, Tumor Cells, Cultured",
author = "Rao, {C N} and V Castronovo and Schmitt, {M C} and Wewer, {U M} and Claysmith, {A P} and Liotta, {L A} and Sobel, {M E}",
year = "1989",
month = sep,
day = "5",
language = "English",
volume = "28",
pages = "7476--86",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "18",

}

RIS

TY - JOUR

T1 - Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor

AU - Rao, C N

AU - Castronovo, V

AU - Schmitt, M C

AU - Wewer, U M

AU - Claysmith, A P

AU - Liotta, L A

AU - Sobel, M E

PY - 1989/9/5

Y1 - 1989/9/5

N2 - The high-affinity cellular receptor for the basement membrane component laminin is differentially expressed during tumor invasion and metastasis. A cDNA clone encoding the murine laminin receptor was isolated and identified on the basis of sequence homology to the human laminin receptor [Wewer et al. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 7137-7141]. Primer extension experiments demonstrated that the clone contained the complete 5' sequence of the murine laminin receptor mRNA. RNA blot data demonstrated a single-sized laminin receptor mRNA, approximately 1400 bases long, in human, mouse, and rat. The nascent laminin receptor predicted from the cDNA sequence is 295 amino acids long, with a molecular weight of 33,000, and contains one intradisulfide bridge, a short putative transmembrane domain, and an extracellular carboxy-terminal region which has abundant glutamic acid residues and multiple repeat sequences. The precursor of the laminin receptor is apparently smaller than the 67-kilodalton protein isolated from tissue. The apparent molecular weight on SDS-polyacrylamide gels of the rabbit reticulocyte cell-free translation product of selectively hybridized laminin receptor mRNA is 37,000. Antisera to three different domains of the cDNA-predicted receptor were used to study the relationship between the 37- and 67-kilodalton polypeptides. Antisera to cDNA-deduced synthetic peptides of the receptor immunoprecipitated a 37-kilodalton band both from cell-free translation products and from pulse-labeled cell extracts. On immunoblots of cell extracts, one antisynthetic peptide antiserum recognized only the 67-kilodalton receptor, while another antiserum identified both 37- and 67-kilodalton polypeptides, suggesting a precursor-product relationship between the two polypeptides.

AB - The high-affinity cellular receptor for the basement membrane component laminin is differentially expressed during tumor invasion and metastasis. A cDNA clone encoding the murine laminin receptor was isolated and identified on the basis of sequence homology to the human laminin receptor [Wewer et al. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 7137-7141]. Primer extension experiments demonstrated that the clone contained the complete 5' sequence of the murine laminin receptor mRNA. RNA blot data demonstrated a single-sized laminin receptor mRNA, approximately 1400 bases long, in human, mouse, and rat. The nascent laminin receptor predicted from the cDNA sequence is 295 amino acids long, with a molecular weight of 33,000, and contains one intradisulfide bridge, a short putative transmembrane domain, and an extracellular carboxy-terminal region which has abundant glutamic acid residues and multiple repeat sequences. The precursor of the laminin receptor is apparently smaller than the 67-kilodalton protein isolated from tissue. The apparent molecular weight on SDS-polyacrylamide gels of the rabbit reticulocyte cell-free translation product of selectively hybridized laminin receptor mRNA is 37,000. Antisera to three different domains of the cDNA-predicted receptor were used to study the relationship between the 37- and 67-kilodalton polypeptides. Antisera to cDNA-deduced synthetic peptides of the receptor immunoprecipitated a 37-kilodalton band both from cell-free translation products and from pulse-labeled cell extracts. On immunoblots of cell extracts, one antisynthetic peptide antiserum recognized only the 67-kilodalton receptor, while another antiserum identified both 37- and 67-kilodalton polypeptides, suggesting a precursor-product relationship between the two polypeptides.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - DNA

KW - Humans

KW - Immunoblotting

KW - Laminin

KW - Mice

KW - Molecular Sequence Data

KW - Neoplasm Metastasis

KW - Nucleic Acid Conformation

KW - Nucleic Acid Hybridization

KW - Protein Biosynthesis

KW - Protein Conformation

KW - Protein Precursors

KW - RNA, Messenger

KW - Rats

KW - Receptors, Immunologic

KW - Receptors, Laminin

KW - Restriction Mapping

KW - Sequence Homology, Nucleic Acid

KW - Tumor Cells, Cultured

M3 - Journal article

C2 - 2531008

VL - 28

SP - 7476

EP - 7486

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 18

ER -

ID: 34330128