Evaluation of a Salmonella‐specific DNA probe by colony hybridization using non‐isotopic and isotopic labeling

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Evaluation of a Salmonella‐specific DNA probe by colony hybridization using non‐isotopic and isotopic labeling. / AABO, S.; THOMAS, A.; HALL, M. L.M.; SMITH, H. R.; OLSEN, J. E.

In: APMIS, Vol. 100, No. 7-12, 07.1992, p. 623-628.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

AABO, S, THOMAS, A, HALL, MLM, SMITH, HR & OLSEN, JE 1992, 'Evaluation of a Salmonella‐specific DNA probe by colony hybridization using non‐isotopic and isotopic labeling', APMIS, vol. 100, no. 7-12, pp. 623-628. https://doi.org/10.1111/j.1699-0463.1992.tb03976.x

APA

AABO, S., THOMAS, A., HALL, M. L. M., SMITH, H. R., & OLSEN, J. E. (1992). Evaluation of a Salmonella‐specific DNA probe by colony hybridization using non‐isotopic and isotopic labeling. APMIS, 100(7-12), 623-628. https://doi.org/10.1111/j.1699-0463.1992.tb03976.x

Vancouver

AABO S, THOMAS A, HALL MLM, SMITH HR, OLSEN JE. Evaluation of a Salmonella‐specific DNA probe by colony hybridization using non‐isotopic and isotopic labeling. APMIS. 1992 Jul;100(7-12):623-628. https://doi.org/10.1111/j.1699-0463.1992.tb03976.x

Author

AABO, S. ; THOMAS, A. ; HALL, M. L.M. ; SMITH, H. R. ; OLSEN, J. E. / Evaluation of a Salmonella‐specific DNA probe by colony hybridization using non‐isotopic and isotopic labeling. In: APMIS. 1992 ; Vol. 100, No. 7-12. pp. 623-628.

Bibtex

@article{2b998155be5e47ae8fc22d79da242cee,
title = "Evaluation of a Salmonella‐specific DNA probe by colony hybridization using non‐isotopic and isotopic labeling",
abstract = "A 2.3 kilobase (kb) Salmonella probe, JEO402‐1, and two subfragments, F1214 (1.3 kb) and F1217 (0.8 kb), have been evaluated by colony hybridization using pure cultures of Salmonella serovars and non‐salmonella bacteria. JEO402‐1, and its subfragments, F1214 and F1217, hybridized to all of 156 different Salmonella serovars tested, while there was no reaction to 112 non‐salmonella strains belonging to 19 genera and 37 species of Enterobacteriaceae. Together with previously published results, the JEO402‐1 probe has now been shown to detect a total of 396 Salmonella strains belonging to 214 serovars of Slamonella subspecies I‐VI. A total of 178 non‐salmonella strains representing 23 genera and 51 species of Enterobacteriaceae have all tested negative with JEO402‐1. The hybridization results obtained using a digoxigenin‐labeled probe were similar to those obtained with 35S isotopic labeling when complete colony lysis was ensured.",
keywords = "S, digoxigenin, DNA probe, Hybridization, Salmonella",
author = "S. AABO and A. THOMAS and HALL, {M. L.M.} and SMITH, {H. R.} and OLSEN, {J. E.}",
year = "1992",
month = jul,
doi = "10.1111/j.1699-0463.1992.tb03976.x",
language = "English",
volume = "100",
pages = "623--628",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "7-12",

}

RIS

TY - JOUR

T1 - Evaluation of a Salmonella‐specific DNA probe by colony hybridization using non‐isotopic and isotopic labeling

AU - AABO, S.

AU - THOMAS, A.

AU - HALL, M. L.M.

AU - SMITH, H. R.

AU - OLSEN, J. E.

PY - 1992/7

Y1 - 1992/7

N2 - A 2.3 kilobase (kb) Salmonella probe, JEO402‐1, and two subfragments, F1214 (1.3 kb) and F1217 (0.8 kb), have been evaluated by colony hybridization using pure cultures of Salmonella serovars and non‐salmonella bacteria. JEO402‐1, and its subfragments, F1214 and F1217, hybridized to all of 156 different Salmonella serovars tested, while there was no reaction to 112 non‐salmonella strains belonging to 19 genera and 37 species of Enterobacteriaceae. Together with previously published results, the JEO402‐1 probe has now been shown to detect a total of 396 Salmonella strains belonging to 214 serovars of Slamonella subspecies I‐VI. A total of 178 non‐salmonella strains representing 23 genera and 51 species of Enterobacteriaceae have all tested negative with JEO402‐1. The hybridization results obtained using a digoxigenin‐labeled probe were similar to those obtained with 35S isotopic labeling when complete colony lysis was ensured.

AB - A 2.3 kilobase (kb) Salmonella probe, JEO402‐1, and two subfragments, F1214 (1.3 kb) and F1217 (0.8 kb), have been evaluated by colony hybridization using pure cultures of Salmonella serovars and non‐salmonella bacteria. JEO402‐1, and its subfragments, F1214 and F1217, hybridized to all of 156 different Salmonella serovars tested, while there was no reaction to 112 non‐salmonella strains belonging to 19 genera and 37 species of Enterobacteriaceae. Together with previously published results, the JEO402‐1 probe has now been shown to detect a total of 396 Salmonella strains belonging to 214 serovars of Slamonella subspecies I‐VI. A total of 178 non‐salmonella strains representing 23 genera and 51 species of Enterobacteriaceae have all tested negative with JEO402‐1. The hybridization results obtained using a digoxigenin‐labeled probe were similar to those obtained with 35S isotopic labeling when complete colony lysis was ensured.

KW - S

KW - digoxigenin

KW - DNA probe

KW - Hybridization

KW - Salmonella

U2 - 10.1111/j.1699-0463.1992.tb03976.x

DO - 10.1111/j.1699-0463.1992.tb03976.x

M3 - Journal article

C2 - 1642849

AN - SCOPUS:0026721211

VL - 100

SP - 623

EP - 628

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 7-12

ER -

ID: 251187205