TY - JOUR

T1 - Enzyme-assisted extraction of rapeseed oil with minimum water addition: a proof-of-concept study

AU - Thomsen, Kristian

AU - Raak, Norbert

AU - Gregersen, Sandra Beyer

AU - Månsson, Lars

AU - Miquel Becker, Eleonora

N1 - Funding information: The work is in collaboration with Dansk Landbrugs Grovvareselskab, Siccadania A/S, Dragsbæk A/S, Funkis Food and DTI and is supported by the Danish Green Development and Demonstration Program (Grønt Udviklings- og DemonstrationsProgram; GUDP). Funding for NR was received from Novo Nordisk Fonden (grant number NNF21OC0071375).

PY - 2024

Y1 - 2024

N2 - The increased demand for vegetable oils calls for new strategies for enhancing the yield of mechanical oil extraction, preferably without the use of potentially hazardous solvents such as n-hexane. Enzyme-assisted oil extraction has been studied under aqueous conditions, where the oil recovery was enhanced due to the partial enzymatic breakdown of cell wall compounds. However, this process is often not feasible for industrial applications, where mechanical pressing of dry seeds is implemented. This study provides a proof-of-concept for enzyme-assisted rapeseed oil extraction with minimum water addition (1 mL per 100 g rapeseeds). The application of cellulytic and pectolytic enzymes increased the reducing sugar content as a result of the carbohydrate breakdown. The oil recovery in the mechanical pressing was increased significantly from ~60% to ~65%, and the quality of the oils was not significantly affected by the enzyme treatment.

AB - The increased demand for vegetable oils calls for new strategies for enhancing the yield of mechanical oil extraction, preferably without the use of potentially hazardous solvents such as n-hexane. Enzyme-assisted oil extraction has been studied under aqueous conditions, where the oil recovery was enhanced due to the partial enzymatic breakdown of cell wall compounds. However, this process is often not feasible for industrial applications, where mechanical pressing of dry seeds is implemented. This study provides a proof-of-concept for enzyme-assisted rapeseed oil extraction with minimum water addition (1 mL per 100 g rapeseeds). The application of cellulytic and pectolytic enzymes increased the reducing sugar content as a result of the carbohydrate breakdown. The oil recovery in the mechanical pressing was increased significantly from ~60% to ~65%, and the quality of the oils was not significantly affected by the enzyme treatment.

U2 - 10.1111/ijfs.17030

DO - 10.1111/ijfs.17030

M3 - Journal article

VL - 59

SP - 3013

EP - 3019

JO - International Journal of Food Science and Technology

JF - International Journal of Food Science and Technology

SN - 0950-5423

IS - 5

ER -