Differential methylation in the GSTT1 regulatory region in sudden unexplained death and sudden unexpected death in epilepsy

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Standard

Differential methylation in the GSTT1 regulatory region in sudden unexplained death and sudden unexpected death in epilepsy. / Christiansen, Steffan Noe; Jacobsen, Stine Bøttcher; Andersen, Jeppe Dyrberg; Kampmann, Marie Louise; Trudsø, Linea Christine; Olsen, Kristine Boisen; Tfelt-Hansen, Jacob; Banner, Jytte; Morling, Niels.

In: International Journal of Molecular Sciences, Vol. 22, No. 6, 2790, 02.03.2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Christiansen, SN, Jacobsen, SB, Andersen, JD, Kampmann, ML, Trudsø, LC, Olsen, KB, Tfelt-Hansen, J, Banner, J & Morling, N 2021, 'Differential methylation in the GSTT1 regulatory region in sudden unexplained death and sudden unexpected death in epilepsy', International Journal of Molecular Sciences, vol. 22, no. 6, 2790. https://doi.org/10.3390/ijms22062790

APA

Christiansen, S. N., Jacobsen, S. B., Andersen, J. D., Kampmann, M. L., Trudsø, L. C., Olsen, K. B., Tfelt-Hansen, J., Banner, J., & Morling, N. (2021). Differential methylation in the GSTT1 regulatory region in sudden unexplained death and sudden unexpected death in epilepsy. International Journal of Molecular Sciences, 22(6), [2790]. https://doi.org/10.3390/ijms22062790

Vancouver

Christiansen SN, Jacobsen SB, Andersen JD, Kampmann ML, Trudsø LC, Olsen KB et al. Differential methylation in the GSTT1 regulatory region in sudden unexplained death and sudden unexpected death in epilepsy. International Journal of Molecular Sciences. 2021 Mar 2;22(6). 2790. https://doi.org/10.3390/ijms22062790

Author

Christiansen, Steffan Noe ; Jacobsen, Stine Bøttcher ; Andersen, Jeppe Dyrberg ; Kampmann, Marie Louise ; Trudsø, Linea Christine ; Olsen, Kristine Boisen ; Tfelt-Hansen, Jacob ; Banner, Jytte ; Morling, Niels. / Differential methylation in the GSTT1 regulatory region in sudden unexplained death and sudden unexpected death in epilepsy. In: International Journal of Molecular Sciences. 2021 ; Vol. 22, No. 6.

Bibtex

@article{ae321e8ea30f47d7958d44646553324c,
title = "Differential methylation in the GSTT1 regulatory region in sudden unexplained death and sudden unexpected death in epilepsy",
abstract = "Sudden cardiac death (SCD) is a diagnostic challenge in forensic medicine. In a relatively large proportion of the SCDs, the deaths remain unexplained after autopsy. This challenge is likely caused by unknown disease mechanisms. Changes in DNA methylation have been associated with several heart diseases, but the role of DNA methylation in SCD is unknown. In this study, we investigated DNA methylation in two SCD subtypes, sudden unexplained death (SUD) and sudden unexpected death in epilepsy (SUDEP). We assessed DNA methylation of more than 850,000 positions in cardiac tissue from nine SUD and 14 SUDEP cases using the Illumina Infinium MethylationEPIC BeadChip. In total, six differently methylated regions (DMRs) between the SUD and SUDEP cases were identified. The DMRs were located in proximity to or overlapping genes encoding proteins that are a part of the glutathione S-transferase (GST) superfamily. Whole genome sequencing (WGS) showed that the DNA methylation alterations were not caused by genetic changes, while whole transcriptome sequencing (WTS) showed that DNA methylation was associated with expression levels of the GSTT1 gene. In conclusion, our results indicate that cardiac DNA methylation is similar in SUD and SUDEP, but with regional differential methylation in proximity to GST genes.",
keywords = "DNA methylation, Epigenetics, Genetics, Molecular autopsy, RNA, Sudden cardiac death, Sudden unexpected death in epilepsy, Sudden unexplained death",
author = "Christiansen, {Steffan Noe} and Jacobsen, {Stine B{\o}ttcher} and Andersen, {Jeppe Dyrberg} and Kampmann, {Marie Louise} and Truds{\o}, {Linea Christine} and Olsen, {Kristine Boisen} and Jacob Tfelt-Hansen and Jytte Banner and Niels Morling",
year = "2021",
month = mar,
day = "2",
doi = "10.3390/ijms22062790",
language = "English",
volume = "22",
journal = "International Journal of Molecular Sciences (CD-ROM)",
issn = "1424-6783",
publisher = "M D P I AG",
number = "6",

}

RIS

TY - JOUR

T1 - Differential methylation in the GSTT1 regulatory region in sudden unexplained death and sudden unexpected death in epilepsy

AU - Christiansen, Steffan Noe

AU - Jacobsen, Stine Bøttcher

AU - Andersen, Jeppe Dyrberg

AU - Kampmann, Marie Louise

AU - Trudsø, Linea Christine

AU - Olsen, Kristine Boisen

AU - Tfelt-Hansen, Jacob

AU - Banner, Jytte

AU - Morling, Niels

PY - 2021/3/2

Y1 - 2021/3/2

N2 - Sudden cardiac death (SCD) is a diagnostic challenge in forensic medicine. In a relatively large proportion of the SCDs, the deaths remain unexplained after autopsy. This challenge is likely caused by unknown disease mechanisms. Changes in DNA methylation have been associated with several heart diseases, but the role of DNA methylation in SCD is unknown. In this study, we investigated DNA methylation in two SCD subtypes, sudden unexplained death (SUD) and sudden unexpected death in epilepsy (SUDEP). We assessed DNA methylation of more than 850,000 positions in cardiac tissue from nine SUD and 14 SUDEP cases using the Illumina Infinium MethylationEPIC BeadChip. In total, six differently methylated regions (DMRs) between the SUD and SUDEP cases were identified. The DMRs were located in proximity to or overlapping genes encoding proteins that are a part of the glutathione S-transferase (GST) superfamily. Whole genome sequencing (WGS) showed that the DNA methylation alterations were not caused by genetic changes, while whole transcriptome sequencing (WTS) showed that DNA methylation was associated with expression levels of the GSTT1 gene. In conclusion, our results indicate that cardiac DNA methylation is similar in SUD and SUDEP, but with regional differential methylation in proximity to GST genes.

AB - Sudden cardiac death (SCD) is a diagnostic challenge in forensic medicine. In a relatively large proportion of the SCDs, the deaths remain unexplained after autopsy. This challenge is likely caused by unknown disease mechanisms. Changes in DNA methylation have been associated with several heart diseases, but the role of DNA methylation in SCD is unknown. In this study, we investigated DNA methylation in two SCD subtypes, sudden unexplained death (SUD) and sudden unexpected death in epilepsy (SUDEP). We assessed DNA methylation of more than 850,000 positions in cardiac tissue from nine SUD and 14 SUDEP cases using the Illumina Infinium MethylationEPIC BeadChip. In total, six differently methylated regions (DMRs) between the SUD and SUDEP cases were identified. The DMRs were located in proximity to or overlapping genes encoding proteins that are a part of the glutathione S-transferase (GST) superfamily. Whole genome sequencing (WGS) showed that the DNA methylation alterations were not caused by genetic changes, while whole transcriptome sequencing (WTS) showed that DNA methylation was associated with expression levels of the GSTT1 gene. In conclusion, our results indicate that cardiac DNA methylation is similar in SUD and SUDEP, but with regional differential methylation in proximity to GST genes.

KW - DNA methylation

KW - Epigenetics

KW - Genetics

KW - Molecular autopsy

KW - RNA

KW - Sudden cardiac death

KW - Sudden unexpected death in epilepsy

KW - Sudden unexplained death

U2 - 10.3390/ijms22062790

DO - 10.3390/ijms22062790

M3 - Journal article

C2 - 33801838

AN - SCOPUS:85102122295

VL - 22

JO - International Journal of Molecular Sciences (CD-ROM)

JF - International Journal of Molecular Sciences (CD-ROM)

SN - 1424-6783

IS - 6

M1 - 2790

ER -

ID: 258440328