Determination of the concentration of igg against the spike receptor-binding domain that predicts the viral neutralizing activity of convalescent plasma and serum against sars-cov-2

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Determination of the concentration of igg against the spike receptor-binding domain that predicts the viral neutralizing activity of convalescent plasma and serum against sars-cov-2. / Santiago, Llipsy; Uranga-Murillo, Iratxe; Arias, Maykel; González-Ramírez, Andrés Manuel; Macías-León, Javier; Moreo, Eduardo; Redrado, Sergio; García-García, Ana; Taleb, Víctor; Lira-Navarrete, Erandi; Hurtado-Guerrero, Ramón; Aguilo, Nacho; Encabo-Berzosa, Maria Del Mar; Hidalgo, Sandra; Galvez, Eva M.; Ramirez-Labrada, Ariel; de Miguel, Diego; Benito, Rafael; Miranda, Patricia; Fernández, Antonio; Domingo, José María; Serrano, Laura; Yuste, Cristina; Villanueva-Saz, Sergio; Paño-Pardo, José Ramón; Pardo, Julián.

In: Biology, Vol. 10, No. 3, 208, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Santiago, L, Uranga-Murillo, I, Arias, M, González-Ramírez, AM, Macías-León, J, Moreo, E, Redrado, S, García-García, A, Taleb, V, Lira-Navarrete, E, Hurtado-Guerrero, R, Aguilo, N, Encabo-Berzosa, MDM, Hidalgo, S, Galvez, EM, Ramirez-Labrada, A, de Miguel, D, Benito, R, Miranda, P, Fernández, A, Domingo, JM, Serrano, L, Yuste, C, Villanueva-Saz, S, Paño-Pardo, JR & Pardo, J 2021, 'Determination of the concentration of igg against the spike receptor-binding domain that predicts the viral neutralizing activity of convalescent plasma and serum against sars-cov-2', Biology, vol. 10, no. 3, 208. https://doi.org/10.3390/biology10030208

APA

Santiago, L., Uranga-Murillo, I., Arias, M., González-Ramírez, A. M., Macías-León, J., Moreo, E., Redrado, S., García-García, A., Taleb, V., Lira-Navarrete, E., Hurtado-Guerrero, R., Aguilo, N., Encabo-Berzosa, M. D. M., Hidalgo, S., Galvez, E. M., Ramirez-Labrada, A., de Miguel, D., Benito, R., Miranda, P., ... Pardo, J. (2021). Determination of the concentration of igg against the spike receptor-binding domain that predicts the viral neutralizing activity of convalescent plasma and serum against sars-cov-2. Biology, 10(3), [208]. https://doi.org/10.3390/biology10030208

Vancouver

Santiago L, Uranga-Murillo I, Arias M, González-Ramírez AM, Macías-León J, Moreo E et al. Determination of the concentration of igg against the spike receptor-binding domain that predicts the viral neutralizing activity of convalescent plasma and serum against sars-cov-2. Biology. 2021;10(3). 208. https://doi.org/10.3390/biology10030208

Author

Santiago, Llipsy ; Uranga-Murillo, Iratxe ; Arias, Maykel ; González-Ramírez, Andrés Manuel ; Macías-León, Javier ; Moreo, Eduardo ; Redrado, Sergio ; García-García, Ana ; Taleb, Víctor ; Lira-Navarrete, Erandi ; Hurtado-Guerrero, Ramón ; Aguilo, Nacho ; Encabo-Berzosa, Maria Del Mar ; Hidalgo, Sandra ; Galvez, Eva M. ; Ramirez-Labrada, Ariel ; de Miguel, Diego ; Benito, Rafael ; Miranda, Patricia ; Fernández, Antonio ; Domingo, José María ; Serrano, Laura ; Yuste, Cristina ; Villanueva-Saz, Sergio ; Paño-Pardo, José Ramón ; Pardo, Julián. / Determination of the concentration of igg against the spike receptor-binding domain that predicts the viral neutralizing activity of convalescent plasma and serum against sars-cov-2. In: Biology. 2021 ; Vol. 10, No. 3.

Bibtex

@article{b1500587dbfd4af790e8ef2fcfe79ba8,
title = "Determination of the concentration of igg against the spike receptor-binding domain that predicts the viral neutralizing activity of convalescent plasma and serum against sars-cov-2",
abstract = "Several hundred millions of people have been diagnosed of coronavirus disease 2019 (COVID-19), causing millions of deaths and a high socioeconomic burden. SARS-CoV-2, the causative agent of COVID-19, induces both specific T-and B-cell responses, being antibodies against the virus detected a few days after infection. Passive immunization with hyperimmune plasma from convalescent patients has been proposed as a potentially useful treatment for COVID-19. Using an in-house quantitative ELISA test, we found that plasma from 177 convalescent donors contained IgG antibodies specific to the spike receptor-binding domain (RBD) of SARS-CoV-2, although at very different concentrations which correlated with previous disease severity and gender. Anti-RBD IgG plasma concentrations significantly correlated with the plasma viral neutralizing activity (VN) against SARS-CoV-2 in vitro. Similar results were found using an independent cohort of serum from 168 convalescent health workers. These results validate an in-house RBD IgG ELISA test in a large cohort of COVID-19 convalescent patients and indicate that plasma from all convalescent donors does not contain a high enough amount of anti-SARS-CoV-2-RBD neutralizing IgG to prevent SARS-CoV-2 infection in vitro. The use of quantitative anti-RBD IgG detection systems might help to predict the efficacy of the passive immunization using plasma from patients recovered from SARS-CoV-2.",
keywords = "Antibody, Convalescent plasma, Coronavirus, ELISA, IgG, SARS-CoV-2",
author = "Llipsy Santiago and Iratxe Uranga-Murillo and Maykel Arias and Gonz{\'a}lez-Ram{\'i}rez, {Andr{\'e}s Manuel} and Javier Mac{\'i}as-Le{\'o}n and Eduardo Moreo and Sergio Redrado and Ana Garc{\'i}a-Garc{\'i}a and V{\'i}ctor Taleb and Erandi Lira-Navarrete and Ram{\'o}n Hurtado-Guerrero and Nacho Aguilo and Encabo-Berzosa, {Maria Del Mar} and Sandra Hidalgo and Galvez, {Eva M.} and Ariel Ramirez-Labrada and {de Miguel}, Diego and Rafael Benito and Patricia Miranda and Antonio Fern{\'a}ndez and Domingo, {Jos{\'e} Mar{\'i}a} and Laura Serrano and Cristina Yuste and Sergio Villanueva-Saz and Pa{\~n}o-Pardo, {Jos{\'e} Ram{\'o}n} and Juli{\'a}n Pardo",
year = "2021",
doi = "10.3390/biology10030208",
language = "English",
volume = "10",
journal = "Biology",
issn = "2079-7737",
publisher = "MDPI AG",
number = "3",

}

RIS

TY - JOUR

T1 - Determination of the concentration of igg against the spike receptor-binding domain that predicts the viral neutralizing activity of convalescent plasma and serum against sars-cov-2

AU - Santiago, Llipsy

AU - Uranga-Murillo, Iratxe

AU - Arias, Maykel

AU - González-Ramírez, Andrés Manuel

AU - Macías-León, Javier

AU - Moreo, Eduardo

AU - Redrado, Sergio

AU - García-García, Ana

AU - Taleb, Víctor

AU - Lira-Navarrete, Erandi

AU - Hurtado-Guerrero, Ramón

AU - Aguilo, Nacho

AU - Encabo-Berzosa, Maria Del Mar

AU - Hidalgo, Sandra

AU - Galvez, Eva M.

AU - Ramirez-Labrada, Ariel

AU - de Miguel, Diego

AU - Benito, Rafael

AU - Miranda, Patricia

AU - Fernández, Antonio

AU - Domingo, José María

AU - Serrano, Laura

AU - Yuste, Cristina

AU - Villanueva-Saz, Sergio

AU - Paño-Pardo, José Ramón

AU - Pardo, Julián

PY - 2021

Y1 - 2021

N2 - Several hundred millions of people have been diagnosed of coronavirus disease 2019 (COVID-19), causing millions of deaths and a high socioeconomic burden. SARS-CoV-2, the causative agent of COVID-19, induces both specific T-and B-cell responses, being antibodies against the virus detected a few days after infection. Passive immunization with hyperimmune plasma from convalescent patients has been proposed as a potentially useful treatment for COVID-19. Using an in-house quantitative ELISA test, we found that plasma from 177 convalescent donors contained IgG antibodies specific to the spike receptor-binding domain (RBD) of SARS-CoV-2, although at very different concentrations which correlated with previous disease severity and gender. Anti-RBD IgG plasma concentrations significantly correlated with the plasma viral neutralizing activity (VN) against SARS-CoV-2 in vitro. Similar results were found using an independent cohort of serum from 168 convalescent health workers. These results validate an in-house RBD IgG ELISA test in a large cohort of COVID-19 convalescent patients and indicate that plasma from all convalescent donors does not contain a high enough amount of anti-SARS-CoV-2-RBD neutralizing IgG to prevent SARS-CoV-2 infection in vitro. The use of quantitative anti-RBD IgG detection systems might help to predict the efficacy of the passive immunization using plasma from patients recovered from SARS-CoV-2.

AB - Several hundred millions of people have been diagnosed of coronavirus disease 2019 (COVID-19), causing millions of deaths and a high socioeconomic burden. SARS-CoV-2, the causative agent of COVID-19, induces both specific T-and B-cell responses, being antibodies against the virus detected a few days after infection. Passive immunization with hyperimmune plasma from convalescent patients has been proposed as a potentially useful treatment for COVID-19. Using an in-house quantitative ELISA test, we found that plasma from 177 convalescent donors contained IgG antibodies specific to the spike receptor-binding domain (RBD) of SARS-CoV-2, although at very different concentrations which correlated with previous disease severity and gender. Anti-RBD IgG plasma concentrations significantly correlated with the plasma viral neutralizing activity (VN) against SARS-CoV-2 in vitro. Similar results were found using an independent cohort of serum from 168 convalescent health workers. These results validate an in-house RBD IgG ELISA test in a large cohort of COVID-19 convalescent patients and indicate that plasma from all convalescent donors does not contain a high enough amount of anti-SARS-CoV-2-RBD neutralizing IgG to prevent SARS-CoV-2 infection in vitro. The use of quantitative anti-RBD IgG detection systems might help to predict the efficacy of the passive immunization using plasma from patients recovered from SARS-CoV-2.

KW - Antibody

KW - Convalescent plasma

KW - Coronavirus

KW - ELISA

KW - IgG

KW - SARS-CoV-2

U2 - 10.3390/biology10030208

DO - 10.3390/biology10030208

M3 - Journal article

C2 - 33801808

AN - SCOPUS:85103046471

VL - 10

JO - Biology

JF - Biology

SN - 2079-7737

IS - 3

M1 - 208

ER -

ID: 259816453