CIA2 and CIA2-LIKE are required for optimal photosynthesis and stress responses in Arabidopsis thaliana
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CIA2 and CIA2-LIKE are required for optimal photosynthesis and stress responses in Arabidopsis thaliana. / Gawroński, Piotr; Burdiak, Paweł; Scharff, Lars B.; Mielecki, Jakub; Górecka, Magdalena; Zaborowska, Magdalena; Leister, Dario; Waszczak, Cezary; Karpiński, Stanisław.
In: Plant Journal, Vol. 105, No. 3, 2021, p. 619-638.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - CIA2 and CIA2-LIKE are required for optimal photosynthesis and stress responses in Arabidopsis thaliana
AU - Gawroński, Piotr
AU - Burdiak, Paweł
AU - Scharff, Lars B.
AU - Mielecki, Jakub
AU - Górecka, Magdalena
AU - Zaborowska, Magdalena
AU - Leister, Dario
AU - Waszczak, Cezary
AU - Karpiński, Stanisław
N1 - This article is protected by copyright. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Chloroplast-to-nucleus retrograde signaling is essential for cell function, acclimation to fluctuating environmental conditions, plant growth and development. The vast majority of chloroplast proteins are nuclear-encoded and must be imported into the organelle after synthesis in the cytoplasm. This import is essential for the development of fully functional chloroplasts. On the other hand, functional chloroplasts act as sensors of environmental changes and can trigger acclimatory responses that influence nuclear gene expression. Signaling via mobile transcription factors (TFs) has been recently recognized as a way of communication between organelles and the nucleus. In this study, we performed a targeted reverse genetic screen to identify dual-localized TFs involved in chloroplast retrograde signaling during stress responses. We found that CHLOROPLAST IMPORT APPARATUS 2 (CIA2) has a functional plastid transit peptide and can be located both in chloroplasts and the nucleus. Further, we found that CIA2, along with its homolog CIA2-like (CIL) are involved in the regulation of Arabidopsis responses to UV-AB, high light, and heat shock. Finally, our results suggest that both CIA2 and CIL are crucial for chloroplast translation. Our results contribute to a deeper understanding of signaling events in the chloroplast-nucleus cross-talk.
AB - Chloroplast-to-nucleus retrograde signaling is essential for cell function, acclimation to fluctuating environmental conditions, plant growth and development. The vast majority of chloroplast proteins are nuclear-encoded and must be imported into the organelle after synthesis in the cytoplasm. This import is essential for the development of fully functional chloroplasts. On the other hand, functional chloroplasts act as sensors of environmental changes and can trigger acclimatory responses that influence nuclear gene expression. Signaling via mobile transcription factors (TFs) has been recently recognized as a way of communication between organelles and the nucleus. In this study, we performed a targeted reverse genetic screen to identify dual-localized TFs involved in chloroplast retrograde signaling during stress responses. We found that CHLOROPLAST IMPORT APPARATUS 2 (CIA2) has a functional plastid transit peptide and can be located both in chloroplasts and the nucleus. Further, we found that CIA2, along with its homolog CIA2-like (CIL) are involved in the regulation of Arabidopsis responses to UV-AB, high light, and heat shock. Finally, our results suggest that both CIA2 and CIL are crucial for chloroplast translation. Our results contribute to a deeper understanding of signaling events in the chloroplast-nucleus cross-talk.
U2 - 10.1111/tpj.15058
DO - 10.1111/tpj.15058
M3 - Journal article
C2 - 33119927
VL - 105
SP - 619
EP - 638
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 3
ER -
ID: 250811718