CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. / Christensen, Jeanette Erbo; Ørding Andreasen, Susanne; Christensen, Jan Pravsgaard; Thomsen, Allan Randrup.

In: International Immunology, Vol. 13, No. 4, 2001, p. 593-600.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Christensen, JE, Ørding Andreasen, S, Christensen, JP & Thomsen, AR 2001, 'CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells', International Immunology, vol. 13, no. 4, pp. 593-600.

APA

Christensen, J. E., Ørding Andreasen, S., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. International Immunology, 13(4), 593-600.

Vancouver

Christensen JE, Ørding Andreasen S, Christensen JP, Thomsen AR. CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. International Immunology. 2001;13(4):593-600.

Author

Christensen, Jeanette Erbo ; Ørding Andreasen, Susanne ; Christensen, Jan Pravsgaard ; Thomsen, Allan Randrup. / CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. In: International Immunology. 2001 ; Vol. 13, No. 4. pp. 593-600.

Bibtex

@article{23383f90df6611ddb5fc000ea68e967b,
title = "CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells",
abstract = "CD8(+) T cells in different activation states have been difficult to identify phenotypically. In this study we have investigated whether Mac-1 (CD11b) expression can be used as a criterion to distinguish between recently activated effector cells and memory cells belonging to the CD8(+) T cell subset. Polyclonal virus-specific effector and memory CD8(+) T cells from lymphocytic choriomeningitis- and vesicular stomatitis virus-infected mice were visualized through staining for intracellular IFN-gamma or binding of MHC-peptide tetramers, and Mac-1 expression was evaluated. Naive T cells and most virus-specific memory CD8(+) T cells express little or no Mac-1 independent of the virus model employed. In contrast, the majority of CD8(+) T cells present during acute infection express a significant level of Mac-1 and, similarly, Mac-1 expression is found on secondary effectors generated in response to viral re-exposure. We therefore suggest that high Mac-1 expression defines a subset of circulating effector cells and that the presence of this marker on antigen-specific CD8(+) T cells signifies recent activation.",
author = "Christensen, {Jeanette Erbo} and {{\O}rding Andreasen}, Susanne and Christensen, {Jan Pravsgaard} and Thomsen, {Allan Randrup}",
note = "Keywords: Acute Disease; Animals; Biological Markers; CD8-Positive T-Lymphocytes; Female; Flow Cytometry; Immunologic Memory; Lymphocyte Activation; Lymphocytic Choriomeningitis; Lymphocytic choriomeningitis virus; Macrophage-1 Antigen; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Transgenic; Rhabdoviridae Infections; Vesicular stomatitis Indiana virus",
year = "2001",
language = "English",
volume = "13",
pages = "593--600",
journal = "International Immunology",
issn = "0953-8178",
publisher = "Oxford University Press",
number = "4",

}

RIS

TY - JOUR

T1 - CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells

AU - Christensen, Jeanette Erbo

AU - Ørding Andreasen, Susanne

AU - Christensen, Jan Pravsgaard

AU - Thomsen, Allan Randrup

N1 - Keywords: Acute Disease; Animals; Biological Markers; CD8-Positive T-Lymphocytes; Female; Flow Cytometry; Immunologic Memory; Lymphocyte Activation; Lymphocytic Choriomeningitis; Lymphocytic choriomeningitis virus; Macrophage-1 Antigen; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Transgenic; Rhabdoviridae Infections; Vesicular stomatitis Indiana virus

PY - 2001

Y1 - 2001

N2 - CD8(+) T cells in different activation states have been difficult to identify phenotypically. In this study we have investigated whether Mac-1 (CD11b) expression can be used as a criterion to distinguish between recently activated effector cells and memory cells belonging to the CD8(+) T cell subset. Polyclonal virus-specific effector and memory CD8(+) T cells from lymphocytic choriomeningitis- and vesicular stomatitis virus-infected mice were visualized through staining for intracellular IFN-gamma or binding of MHC-peptide tetramers, and Mac-1 expression was evaluated. Naive T cells and most virus-specific memory CD8(+) T cells express little or no Mac-1 independent of the virus model employed. In contrast, the majority of CD8(+) T cells present during acute infection express a significant level of Mac-1 and, similarly, Mac-1 expression is found on secondary effectors generated in response to viral re-exposure. We therefore suggest that high Mac-1 expression defines a subset of circulating effector cells and that the presence of this marker on antigen-specific CD8(+) T cells signifies recent activation.

AB - CD8(+) T cells in different activation states have been difficult to identify phenotypically. In this study we have investigated whether Mac-1 (CD11b) expression can be used as a criterion to distinguish between recently activated effector cells and memory cells belonging to the CD8(+) T cell subset. Polyclonal virus-specific effector and memory CD8(+) T cells from lymphocytic choriomeningitis- and vesicular stomatitis virus-infected mice were visualized through staining for intracellular IFN-gamma or binding of MHC-peptide tetramers, and Mac-1 expression was evaluated. Naive T cells and most virus-specific memory CD8(+) T cells express little or no Mac-1 independent of the virus model employed. In contrast, the majority of CD8(+) T cells present during acute infection express a significant level of Mac-1 and, similarly, Mac-1 expression is found on secondary effectors generated in response to viral re-exposure. We therefore suggest that high Mac-1 expression defines a subset of circulating effector cells and that the presence of this marker on antigen-specific CD8(+) T cells signifies recent activation.

M3 - Journal article

C2 - 11282998

VL - 13

SP - 593

EP - 600

JO - International Immunology

JF - International Immunology

SN - 0953-8178

IS - 4

ER -

ID: 9639452