Benzyl alcohol induces a reversible fragmentation of the Golgi apparatus and inhibits membrane trafficking between endosomes and the trans-Golgi network
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Benzyl alcohol induces a reversible fragmentation of the Golgi apparatus and inhibits membrane trafficking between endosomes and the trans-Golgi network. / Simm, Roger; Kvalvaag, Audun Sverre; van Deurs, Bo; Lindbäck, Toril; Sandvig, Kirsten.
In: Experimental Cell Research, Vol. 357, No. 1, 01.08.2017, p. 67-78.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Benzyl alcohol induces a reversible fragmentation of the Golgi apparatus and inhibits membrane trafficking between endosomes and the trans-Golgi network
AU - Simm, Roger
AU - Kvalvaag, Audun Sverre
AU - van Deurs, Bo
AU - Lindbäck, Toril
AU - Sandvig, Kirsten
N1 - Copyright © 2017 Elsevier Inc. All rights reserved.
PY - 2017/8/1
Y1 - 2017/8/1
N2 - Benzyl alcohol (BnOH) is widely used as a component of foods, cosmetics, household products and medical products. It is generally considered to be safe for human use, however, it has been connected to a number of adverse effects, including hypersensitivity reactions and neonatal deaths. BnOH is a membrane fluidizing agent that can affect membrane protein activity and cellular processes such as ligand binding to cell surface receptors, endocytosis and degradation of lysosomal cargo. In this study, we examined the effects of BnOH on intracellular transport using Shiga toxin (Stx), diphtheria toxin (DT) and ricin. BnOH caused reduced toxicity of all three toxins at BnOH concentrations that cause membrane fluidization. The reduced toxicity of Stx and ricin was mainly due to inhibition of retrograde transport between endosomes and the trans-Golgi network as BnOH had small effects on cell association and endocytosis of ricin and Stx. Strikingly, BnOH also induced a reversible fragmentation of the Golgi apparatus.
AB - Benzyl alcohol (BnOH) is widely used as a component of foods, cosmetics, household products and medical products. It is generally considered to be safe for human use, however, it has been connected to a number of adverse effects, including hypersensitivity reactions and neonatal deaths. BnOH is a membrane fluidizing agent that can affect membrane protein activity and cellular processes such as ligand binding to cell surface receptors, endocytosis and degradation of lysosomal cargo. In this study, we examined the effects of BnOH on intracellular transport using Shiga toxin (Stx), diphtheria toxin (DT) and ricin. BnOH caused reduced toxicity of all three toxins at BnOH concentrations that cause membrane fluidization. The reduced toxicity of Stx and ricin was mainly due to inhibition of retrograde transport between endosomes and the trans-Golgi network as BnOH had small effects on cell association and endocytosis of ricin and Stx. Strikingly, BnOH also induced a reversible fragmentation of the Golgi apparatus.
KW - Benzyl Alcohol
KW - Biological Transport
KW - Cell Movement
KW - Endocytosis
KW - Endosomes
KW - Golgi Apparatus
KW - HeLa Cells
KW - Humans
KW - Protein Transport
KW - Shiga Toxin
KW - trans-Golgi Network
KW - Journal Article
U2 - 10.1016/j.yexcr.2017.04.025
DO - 10.1016/j.yexcr.2017.04.025
M3 - Journal article
C2 - 28450044
VL - 357
SP - 67
EP - 78
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 1
ER -
ID: 185901272