BAY K 8644-induced oscillations in rabbit gall-bladder transepithelial potential difference.
Research output: Contribution to journal › Journal article › Research › peer-review
The effects of the Ca2+-channel activator BAY K 8644 (a novel dihydropyridine) on transepithelial potential difference (Pd), electrical resistance (Rt), and unidirectional Na+-fluxes were studied in the rabbit gall-bladder. It was observed that BAY K 8644 at concentrations between 10(-7) and 10(-5) M induced regular oscillations in the transepithelial Pd, without affecting the mean value of Pd (or Rt). The mean oscillatory frequency was 18 mHz (approximately 1 cycle per min), and the mean amplitude was 30-35 microV. Oscillations were predominantly elicited from the serosal side. 10(-5) M BAY K 8644 reduced net Na+ -absorption by 16% by inhibiting the mucosa-to-serosa flux. Nifedipine blocked the Pd-oscillations but did not reverse the Na+-transport inhibition. The observed effects of BAY K 8644 are consistent with activation of Ca2+-channels and an increase in intracellular Ca2+-concentration.
Original language | English |
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Journal | Pflügers Archiv: European Journal of Physiology |
Volume | 406 |
Issue number | 5 |
Pages (from-to) | 497-501 |
Number of pages | 4 |
ISSN | 0031-6768 |
Publication status | Published - 1986 |
Bibliographical note
Keywords: 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; Animals; Electric Conductivity; Epithelium; Female; Gallbladder; Nifedipine; Rabbits; Sodium
ID: 8440391