Anti-staphylococcal effects of Myracrodruon urundeuva lectins on nonresistant and multidrug resistant isolates

Research output: Contribution to journal › Journal article › Research › peer-review

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Anti-staphylococcal effects of Myracrodruon urundeuva lectins on nonresistant and multidrug resistant isolates. / Moura, M. C.; Procópio, T. F.; Ferreira, G. R.S.; Alves, R. R.V.; Sá, R. A.; Paiva, P. M.G.; Ingmer, H.; Coelho, L. C.B.B.; Napoleão, T. H.

In: Journal of Applied Microbiology, Vol. 130, No. 3, 2021, p. 745-754.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Moura, MC, Procópio, TF, Ferreira, GRS, Alves, RRV, Sá, RA, Paiva, PMG, Ingmer, H, Coelho, LCBB & Napoleão, TH 2021, 'Anti-staphylococcal effects of Myracrodruon urundeuva lectins on nonresistant and multidrug resistant isolates', Journal of Applied Microbiology, vol. 130, no. 3, pp. 745-754. https://doi.org/10.1111/jam.14811

APA

Moura, M. C., Procópio, T. F., Ferreira, G. R. S., Alves, R. R. V., Sá, R. A., Paiva, P. M. G., Ingmer, H., Coelho, L. C. B. B., & Napoleão, T. H. (2021). Anti-staphylococcal effects of Myracrodruon urundeuva lectins on nonresistant and multidrug resistant isolates. Journal of Applied Microbiology, 130(3), 745-754. https://doi.org/10.1111/jam.14811

Vancouver

Moura MC, Procópio TF, Ferreira GRS, Alves RRV, Sá RA, Paiva PMG et al. Anti-staphylococcal effects of Myracrodruon urundeuva lectins on nonresistant and multidrug resistant isolates. Journal of Applied Microbiology. 2021;130(3):745-754. https://doi.org/10.1111/jam.14811

Author

Moura, M. C. ; Procópio, T. F. ; Ferreira, G. R.S. ; Alves, R. R.V. ; Sá, R. A. ; Paiva, P. M.G. ; Ingmer, H. ; Coelho, L. C.B.B. ; Napoleão, T. H. / Anti-staphylococcal effects of Myracrodruon urundeuva lectins on nonresistant and multidrug resistant isolates. In: Journal of Applied Microbiology. 2021 ; Vol. 130, No. 3. pp. 745-754.

Bibtex

@article{5f5806343e464e5983020169013a3f4d,
title = "Anti-staphylococcal effects of Myracrodruon urundeuva lectins on nonresistant and multidrug resistant isolates",
abstract = "Aims: To evaluate the anti-staphylococcal effects of lectins isolated from bark (MuBL), heartwood (MuHL) and leaves (MuLL) of Myracrodruon urundeuva. Methods and Results: The lectins were evaluated for: effects on growth, aggregation, haemolytic activity and biofilm-forming ability of Staphylococcus aureus clinical isolates nonresistant (8325-4) and multidrug resistant (LAC USA300); interference with the expression of virulence genes (hla, rnaIII and spa) of the Agr system of S. aureus; and synergistic effect with the antibiotics cefoxitin and cefotaxime. MuBL, MuHL and MuLL reduced growth (minimal inhibitory concentration (MIC): 12·5–50 µg ml−1) and viability (minimal bactericidal concentration (MBC): 100 µg ml−1) of 8325-4 and LAC USA300 cells. MuLL (at ½MIC and MIC) reduced LAC USA300 agglutination. The lectins did not interfere with haemolytic activity and expression of hla, rnaIII and spa genes. Only MuHL was able to reduce the biofilm production by 8325-4 (50–400 µg ml−1) and LAC USA300 (400 µg ml−1). Conclusion: The M. urundeuva lectins showed antibacterial activity against nonresistant and resistant clinical isolates of S. aureus and synergistic effects with antibiotics in reducing growth and biofilm formation. Significance and Impact of the Study: This work reports bioactive molecules capable of acting as anti-staphylococcal agents, since there are increasing reports of multiresistant isolates of this bacterium.",
keywords = "antibacterial activity, lectin, multidrug resistant, Staphylococcus aureus, synergistic effects, virulence factors",
author = "Moura, {M. C.} and Proc{\'o}pio, {T. F.} and Ferreira, {G. R.S.} and Alves, {R. R.V.} and S{\'a}, {R. A.} and Paiva, {P. M.G.} and H. Ingmer and Coelho, {L. C.B.B.} and Napole{\~a}o, {T. H.}",
year = "2021",
doi = "10.1111/jam.14811",
language = "English",
volume = "130",
pages = "745--754",
journal = "Proceedings of the Society for Applied Bacteriology",
issn = "0370-1778",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Anti-staphylococcal effects of Myracrodruon urundeuva lectins on nonresistant and multidrug resistant isolates

AU - Moura, M. C.

AU - Procópio, T. F.

AU - Ferreira, G. R.S.

AU - Alves, R. R.V.

AU - Sá, R. A.

AU - Paiva, P. M.G.

AU - Ingmer, H.

AU - Coelho, L. C.B.B.

AU - Napoleão, T. H.

PY - 2021

Y1 - 2021

N2 - Aims: To evaluate the anti-staphylococcal effects of lectins isolated from bark (MuBL), heartwood (MuHL) and leaves (MuLL) of Myracrodruon urundeuva. Methods and Results: The lectins were evaluated for: effects on growth, aggregation, haemolytic activity and biofilm-forming ability of Staphylococcus aureus clinical isolates nonresistant (8325-4) and multidrug resistant (LAC USA300); interference with the expression of virulence genes (hla, rnaIII and spa) of the Agr system of S. aureus; and synergistic effect with the antibiotics cefoxitin and cefotaxime. MuBL, MuHL and MuLL reduced growth (minimal inhibitory concentration (MIC): 12·5–50 µg ml−1) and viability (minimal bactericidal concentration (MBC): 100 µg ml−1) of 8325-4 and LAC USA300 cells. MuLL (at ½MIC and MIC) reduced LAC USA300 agglutination. The lectins did not interfere with haemolytic activity and expression of hla, rnaIII and spa genes. Only MuHL was able to reduce the biofilm production by 8325-4 (50–400 µg ml−1) and LAC USA300 (400 µg ml−1). Conclusion: The M. urundeuva lectins showed antibacterial activity against nonresistant and resistant clinical isolates of S. aureus and synergistic effects with antibiotics in reducing growth and biofilm formation. Significance and Impact of the Study: This work reports bioactive molecules capable of acting as anti-staphylococcal agents, since there are increasing reports of multiresistant isolates of this bacterium.

AB - Aims: To evaluate the anti-staphylococcal effects of lectins isolated from bark (MuBL), heartwood (MuHL) and leaves (MuLL) of Myracrodruon urundeuva. Methods and Results: The lectins were evaluated for: effects on growth, aggregation, haemolytic activity and biofilm-forming ability of Staphylococcus aureus clinical isolates nonresistant (8325-4) and multidrug resistant (LAC USA300); interference with the expression of virulence genes (hla, rnaIII and spa) of the Agr system of S. aureus; and synergistic effect with the antibiotics cefoxitin and cefotaxime. MuBL, MuHL and MuLL reduced growth (minimal inhibitory concentration (MIC): 12·5–50 µg ml−1) and viability (minimal bactericidal concentration (MBC): 100 µg ml−1) of 8325-4 and LAC USA300 cells. MuLL (at ½MIC and MIC) reduced LAC USA300 agglutination. The lectins did not interfere with haemolytic activity and expression of hla, rnaIII and spa genes. Only MuHL was able to reduce the biofilm production by 8325-4 (50–400 µg ml−1) and LAC USA300 (400 µg ml−1). Conclusion: The M. urundeuva lectins showed antibacterial activity against nonresistant and resistant clinical isolates of S. aureus and synergistic effects with antibiotics in reducing growth and biofilm formation. Significance and Impact of the Study: This work reports bioactive molecules capable of acting as anti-staphylococcal agents, since there are increasing reports of multiresistant isolates of this bacterium.

KW - antibacterial activity

KW - lectin

KW - multidrug resistant

KW - Staphylococcus aureus

KW - synergistic effects

KW - virulence factors

U2 - 10.1111/jam.14811

DO - 10.1111/jam.14811

M3 - Journal article

C2 - 32750211

AN - SCOPUS:85089379590

VL - 130

SP - 745

EP - 754

JO - Proceedings of the Society for Applied Bacteriology

JF - Proceedings of the Society for Applied Bacteriology

SN - 0370-1778

IS - 3

ER -

ID: 247339664