17β-Estradiol induces nongenomic effects in renal intercalated cells through G protein-coupled estrogen receptor 1
Research output: Contribution to journal › Journal article › Research › peer-review
Steroid hormones such as 17β-estradiol (E2) are known to modulate ion transporter expression in the kidney through classic intracellular receptors. Steroid hormones are also known to cause rapid nongenomic responses in a variety of nonrenal tissues. However, little is known about renal short-term effects of steroid hormones. Here, we studied the acute actions of E2 on intracellular Ca(2+) signaling in isolated distal convoluted tubules (DCT2), connecting tubules (CNT), and initial cortical collecting ducts (iCCD) by fluo 4 fluorometry. Physiological concentrations of E2 induced transient increases in intracellular Ca(2+) concentration ([Ca(2+)](i)) in a subpopulation of cells. The [Ca(2+)](i) increases required extracellular Ca(2+) and were inhibited by Gd(3+). Strikingly, the classic E2 receptor antagonist ICI 182,780 also increased [Ca(2+)](i), which is inconsistent with the activation of classic E2 receptors. G protein-coupled estrogen receptor 1 (GPER1 or GPR30) was detected in microdissected DCT2/CNT/iCCD by RT-PCR. Stimulation with the specific GPER1 agonist G-1 induced similar [Ca(2+)](i) increases as E2, and in tubules from GPER1 knockout mice, E2, G-1, and ICI 182,780 failed to induce [Ca(2+)](i) elevations. The intercalated cells showed both E2-induced concanamycin-sensitive H(+)-ATPase activity by BCECF fluorometry and the E2-mediated [Ca(2+)](i) increment. We propose that E2 via GPER1 evokes [Ca(2+)](i) transients and increases H(+)-ATPase activity in intercalated cells in mouse DCT2/CNT/iCCD.
Original language | English |
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Journal | A J P: Renal Physiology (Online) |
Volume | 302 |
Issue number | 3 |
Pages (from-to) | F358-68 |
ISSN | 1522-1466 |
DOIs | |
Publication status | Published - 1 Feb 2012 |
Externally published | Yes |
- Aldosterone, Animals, Calcium, Calcium Signaling, Estradiol, Estrogen Receptor alpha, Estrogens, Extracellular Space, Female, Immunohistochemistry, Kidney Tubules, Collecting, Kidney Tubules, Distal, Male, Mice, Mice, Knockout, Microscopy, Immunoelectron, Proton-Translocating ATPases, RNA, Messenger, Receptors, G-Protein-Coupled
Research areas
ID: 138141212