Probiotics and the immunological response to infant vaccinations; a double-blind randomized controlled trial
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Probiotics and the immunological response to infant vaccinations; a double-blind randomized controlled trial. / Sørensen, Camilla Adler; Fuglsang, Eva; Jørgensen, Charlotte Sværke; Laursen, Rikke Pilmann; Larnkjær, Anni; Mølgaard, Christian; Ritz, Christian; Michaelsen, Kim F.; Krogfelt, Karen Angeliki; Frøkiær, Hanne.
In: Clinical Microbiology and Infection, Vol. 25, No. 4, 2019, p. 511.e1-511.e7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Probiotics and the immunological response to infant vaccinations; a double-blind randomized controlled trial
AU - Sørensen, Camilla Adler
AU - Fuglsang, Eva
AU - Jørgensen, Charlotte Sværke
AU - Laursen, Rikke Pilmann
AU - Larnkjær, Anni
AU - Mølgaard, Christian
AU - Ritz, Christian
AU - Michaelsen, Kim F.
AU - Krogfelt, Karen Angeliki
AU - Frøkiær, Hanne
N1 - CURIS 2018 NEXS 317
PY - 2019
Y1 - 2019
N2 - Objectives: To examine the effect of a combination of probiotics on the antibody response to pneumococcal and pertussis vaccination in Danish healthy children, aged 8-14 months, at the time of starting day care. Moreover, the cytokine response to LPS of whole blood was assessed.Methods: A total of 290 children were randomly allocated to receive a combination of Bifidobacterium animalis ssp. lactis and Lactobacillus rhamnosus GG daily for a six month intervention period, and blood samples were drawn at start and end of the study. Specific antibody response towards Streptococcus pneumoniae serotypes and Bordetella pertussis toxin, as well as endotoxin induced IL-6 and IFN-γ production in blood were analysed by Luminex and ELISA.Results: There was no significant difference between the average individual changes (AIC) from baseline to end of study in antibody concentrations for S. pneumoniae for both the probiotics (340.4% ± 11.2%) and the placebo group (382.9% ± 10.4%) (p = 0.525), nor for B. pertussis toxin in the two groups (probiotics 190.1% ± 12.6% vs placebo 238.8% ± 1.1%, p = 0.340). The AIC in IL-6 concentration was significantly lower in the probiotics vs. placebo group (2.9% ± 10.3% vs. 33.7% ± 9.0%, p = 0.024), whereas there was no difference in IFN-γ concentration (0.0% ± 0.2% vs. -0.2% ± 0.1%, p = 0.279).Conclusions: The probiotic intervention did not affect the antibody response against S. pneumoniae and B. pertussis toxin in healthy Danish children.
AB - Objectives: To examine the effect of a combination of probiotics on the antibody response to pneumococcal and pertussis vaccination in Danish healthy children, aged 8-14 months, at the time of starting day care. Moreover, the cytokine response to LPS of whole blood was assessed.Methods: A total of 290 children were randomly allocated to receive a combination of Bifidobacterium animalis ssp. lactis and Lactobacillus rhamnosus GG daily for a six month intervention period, and blood samples were drawn at start and end of the study. Specific antibody response towards Streptococcus pneumoniae serotypes and Bordetella pertussis toxin, as well as endotoxin induced IL-6 and IFN-γ production in blood were analysed by Luminex and ELISA.Results: There was no significant difference between the average individual changes (AIC) from baseline to end of study in antibody concentrations for S. pneumoniae for both the probiotics (340.4% ± 11.2%) and the placebo group (382.9% ± 10.4%) (p = 0.525), nor for B. pertussis toxin in the two groups (probiotics 190.1% ± 12.6% vs placebo 238.8% ± 1.1%, p = 0.340). The AIC in IL-6 concentration was significantly lower in the probiotics vs. placebo group (2.9% ± 10.3% vs. 33.7% ± 9.0%, p = 0.024), whereas there was no difference in IFN-γ concentration (0.0% ± 0.2% vs. -0.2% ± 0.1%, p = 0.279).Conclusions: The probiotic intervention did not affect the antibody response against S. pneumoniae and B. pertussis toxin in healthy Danish children.
KW - Faculty of Science
KW - Cytokine activation
KW - Pertussis
KW - Placebo-controlled study
KW - Pneumococcus
KW - Probiotics
KW - Vaccination response
U2 - 10.1016/j.cmi.2018.07.031
DO - 10.1016/j.cmi.2018.07.031
M3 - Journal article
C2 - 30099133
VL - 25
SP - 511.e1-511.e7
JO - Clinical Microbiology and Infection
JF - Clinical Microbiology and Infection
SN - 1198-743X
IS - 4
ER -
ID: 201039134