Identifying the heterotrimeric complex stoichiometry of AMPK in skeletal muscle by immunoprecipitation
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Identifying the heterotrimeric complex stoichiometry of AMPK in skeletal muscle by immunoprecipitation. / Birk, Jesper Bratz; Wojtaszewski, Jørgen.
AMPK: Methods and Protecols. ed. / Dietbert Neumann; Benoit Viollet. New York : Humana Press, 2018. p. 203-213 (Methods in Molecular Biology; No. 1732).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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TY - CHAP
T1 - Identifying the heterotrimeric complex stoichiometry of AMPK in skeletal muscle by immunoprecipitation
AU - Birk, Jesper Bratz
AU - Wojtaszewski, Jørgen
N1 - CURIS 2018 NEXS 079
PY - 2018
Y1 - 2018
N2 - The 5'-AMP-activated protein kinase is a complicated enzyme consisting of three different subunits, each of which is expressed as two or three isoforms. This gives the possibility of 12 different heterotrimeric complexes, which could have diverse functions within the cell. To map out which of these complexes are present and to what extent in skeletal muscle, we have used the immunoprecipitation technique and analyzed both the precipitates and the remaining supernatants for coprecipitation of complex partners. We have fine-tuned this method to give the best results on lysates from the skeletal muscle, liver, and heart muscle from mouse to man.
AB - The 5'-AMP-activated protein kinase is a complicated enzyme consisting of three different subunits, each of which is expressed as two or three isoforms. This gives the possibility of 12 different heterotrimeric complexes, which could have diverse functions within the cell. To map out which of these complexes are present and to what extent in skeletal muscle, we have used the immunoprecipitation technique and analyzed both the precipitates and the remaining supernatants for coprecipitation of complex partners. We have fine-tuned this method to give the best results on lysates from the skeletal muscle, liver, and heart muscle from mouse to man.
KW - Faculty of Science
KW - AMPK
KW - Homogenization
KW - Protein interaction
KW - Immunoprecipitation
KW - Western blot
KW - In vitro setting
KW - Antibody specificity
U2 - 10.1007/978-1-4939-7598-3_13
DO - 10.1007/978-1-4939-7598-3_13
M3 - Book chapter
C2 - 29480477
SN - 978-1-4939-7597-6
T3 - Methods in Molecular Biology
SP - 203
EP - 213
BT - AMPK
A2 - Neumann, Dietbert
A2 - Viollet, Benoit
PB - Humana Press
CY - New York
ER -
ID: 191293422