Determinants of maximal whole-body fat oxidation in elite cross-country skiers: Role of skeletal muscle mitochondria
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Determinants of maximal whole-body fat oxidation in elite cross-country skiers : Role of skeletal muscle mitochondria. / Dandanell, Sune; Meinild-Lundby, Anne-Kristine; Andersen, Andreas Breenfeldt; Lang, Paul F; Oberholzer, Laura; Keiser, Stefanie; Robach, Paul; Larsen, Steen; Rønnestad, Bent R; Lundby, Carsten.
In: Scandinavian Journal of Medicine & Science in Sports, Vol. 28, No. 12, 2018, p. 2494-2504.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Determinants of maximal whole-body fat oxidation in elite cross-country skiers
T2 - Role of skeletal muscle mitochondria
AU - Dandanell, Sune
AU - Meinild-Lundby, Anne-Kristine
AU - Andersen, Andreas Breenfeldt
AU - Lang, Paul F
AU - Oberholzer, Laura
AU - Keiser, Stefanie
AU - Robach, Paul
AU - Larsen, Steen
AU - Rønnestad, Bent R
AU - Lundby, Carsten
N1 - (ekstern)
PY - 2018
Y1 - 2018
N2 - Elite endurance athletes possess a high capacity for whole-body maximal fat oxidation (MFO). The aim was to investigate the determinants of a high MFO in endurance athletes. The hypotheses were that augmented MFO in endurance athletes is related to concomitantly increments of skeletal muscle mitochondrial volume density (MitoVD ) and mitochondrial fatty acid oxidation (FAOp ), that is, quantitative mitochondrial adaptations as well as intrinsic FAOp per mitochondria, that is, qualitative adaptations. Eight competitive male cross-country skiers and eight untrained controls were compared in the study. A graded exercise test was performed to determine MFO, the intensity where MFO occurs (FatMax ), and V˙O2Max . Skeletal muscle biopsies were obtained to determine MitoVD (electron microscopy), FAOp , and OXPHOSp (high-resolution respirometry). The following were higher (P < 0.05) in endurance athletes compared to controls: MFO (mean [95% confidence intervals]) (0.60 g/min [0.50-0.70] vs 0.32 [0.24-0.39]), FatMax (46% V˙O2Max [44-47] vs 35 [34-37]), V˙ O2Max (71 mL/min/kg [69-72] vs 48 [47-49]), MitoVD (7.8% [7.2-8.5] vs 6.0 [5.3-6.8]), FAOp (34 pmol/s/mg muscle ww [27-40] vs 21 [17-25]), and OXPHOSp (108 pmol/s/mg muscle ww [104-112] vs 69 [68-71]). Intrinsic FAOp (4.0 pmol/s/mg muscle w.w/MitoVD [2.7-5.3] vs 3.3 [2.7-3.9]) and OXPHOSp (14 pmol/s/mg muscle ww/MitoVD [13-15] vs 11 [10-13]) were, however, similar in the endurance athletes and untrained controls. MFO and MitoVD correlated (r2 = 0.504, P < 0.05) in the endurance athletes. A strong correlation between MitoVD and MFO suggests that expansion of MitoVD might be rate-limiting for MFO in the endurance athletes. In contrast, intrinsic mitochondrial changes were not associated with augmented MFO.
AB - Elite endurance athletes possess a high capacity for whole-body maximal fat oxidation (MFO). The aim was to investigate the determinants of a high MFO in endurance athletes. The hypotheses were that augmented MFO in endurance athletes is related to concomitantly increments of skeletal muscle mitochondrial volume density (MitoVD ) and mitochondrial fatty acid oxidation (FAOp ), that is, quantitative mitochondrial adaptations as well as intrinsic FAOp per mitochondria, that is, qualitative adaptations. Eight competitive male cross-country skiers and eight untrained controls were compared in the study. A graded exercise test was performed to determine MFO, the intensity where MFO occurs (FatMax ), and V˙O2Max . Skeletal muscle biopsies were obtained to determine MitoVD (electron microscopy), FAOp , and OXPHOSp (high-resolution respirometry). The following were higher (P < 0.05) in endurance athletes compared to controls: MFO (mean [95% confidence intervals]) (0.60 g/min [0.50-0.70] vs 0.32 [0.24-0.39]), FatMax (46% V˙O2Max [44-47] vs 35 [34-37]), V˙ O2Max (71 mL/min/kg [69-72] vs 48 [47-49]), MitoVD (7.8% [7.2-8.5] vs 6.0 [5.3-6.8]), FAOp (34 pmol/s/mg muscle ww [27-40] vs 21 [17-25]), and OXPHOSp (108 pmol/s/mg muscle ww [104-112] vs 69 [68-71]). Intrinsic FAOp (4.0 pmol/s/mg muscle w.w/MitoVD [2.7-5.3] vs 3.3 [2.7-3.9]) and OXPHOSp (14 pmol/s/mg muscle ww/MitoVD [13-15] vs 11 [10-13]) were, however, similar in the endurance athletes and untrained controls. MFO and MitoVD correlated (r2 = 0.504, P < 0.05) in the endurance athletes. A strong correlation between MitoVD and MFO suggests that expansion of MitoVD might be rate-limiting for MFO in the endurance athletes. In contrast, intrinsic mitochondrial changes were not associated with augmented MFO.
KW - Faculty of Science
KW - Cross-country skiing
KW - Endurance training
KW - Intrinsic mitochondrial function
KW - Mitochondrial fat oxidation
KW - Mitochondrial volume density
U2 - 10.1111/sms.13298
DO - 10.1111/sms.13298
M3 - Journal article
C2 - 30218613
VL - 28
SP - 2494
EP - 2504
JO - Scandinavian Journal of Medicine & Science in Sports
JF - Scandinavian Journal of Medicine & Science in Sports
SN - 0905-7188
IS - 12
ER -
ID: 203897022