Discovery of urinary biomarkers of spinach consumption using untargeted LC-MS metabolomics in a human intervention trial
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Discovery of urinary biomarkers of spinach consumption using untargeted LC-MS metabolomics in a human intervention trial. / Xi, Muyao; La Barbera, Giorgia; Eriksen, Jane Nygaard; Prahm, August Pilegaard; Jeppesen, Palle Bekker; Dragsted, Lars Ove.
In: Molecular Nutrition & Food Research, Vol. 66, No. 6, 2100260, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Discovery of urinary biomarkers of spinach consumption using untargeted LC-MS metabolomics in a human intervention trial
AU - Xi, Muyao
AU - La Barbera, Giorgia
AU - Eriksen, Jane Nygaard
AU - Prahm, August Pilegaard
AU - Jeppesen, Palle Bekker
AU - Dragsted, Lars Ove
N1 - This article is protected by copyright. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Scope: Biomarkers for intake of green leafy vegetables such as spinach can help investigate their health effects. However, only few potential intake markers have been reported in the literature so far.Methods and results: Based on a cross-over study on whole leaf and minced spinach, we investigate changes in metabolites before and after spinach intake and differences between the two treatments and health status. Nineteen volunteers (12 healthy subjects and 7 short bowel patients) completed the study within 48 days. Urine samples (24-h intervals before and after spinach intake) and serum samples (baseline, post 8 d, and post 15 d) are collected and analyzed by ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS). The acquired data is analyzed by multivariate and univariate analyses. Three candidate biomarkers are observed in urine only after the spinach intake, including des-amino arginine pentenol ester, D/L-malic acid ester of cis-p-coumarate, D/L-malic acid ester of trans-p-coumarate, and 69 metabolites are present before spinach intake but showing an altered level after treatment. These metabolites are related to dietary habits or meal structure, and some changes are possibly affected by spinach intake. The candidate biomarkers are independent of spinach pre-processing and healthy status. No markers are discovered in serum samples.Conclusion: We propose structures for three candidate spinach intake biomarkers; these markers will need further validation in independent studies. This article is protected by copyright. All rights reserved.
AB - Scope: Biomarkers for intake of green leafy vegetables such as spinach can help investigate their health effects. However, only few potential intake markers have been reported in the literature so far.Methods and results: Based on a cross-over study on whole leaf and minced spinach, we investigate changes in metabolites before and after spinach intake and differences between the two treatments and health status. Nineteen volunteers (12 healthy subjects and 7 short bowel patients) completed the study within 48 days. Urine samples (24-h intervals before and after spinach intake) and serum samples (baseline, post 8 d, and post 15 d) are collected and analyzed by ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS). The acquired data is analyzed by multivariate and univariate analyses. Three candidate biomarkers are observed in urine only after the spinach intake, including des-amino arginine pentenol ester, D/L-malic acid ester of cis-p-coumarate, D/L-malic acid ester of trans-p-coumarate, and 69 metabolites are present before spinach intake but showing an altered level after treatment. These metabolites are related to dietary habits or meal structure, and some changes are possibly affected by spinach intake. The candidate biomarkers are independent of spinach pre-processing and healthy status. No markers are discovered in serum samples.Conclusion: We propose structures for three candidate spinach intake biomarkers; these markers will need further validation in independent studies. This article is protected by copyright. All rights reserved.
KW - Faculty of Science
KW - Spinach
KW - Biomarkers of intake
KW - LC-MS
KW - Untargeted metabolomics
KW - Effect biomarkers
U2 - 10.1002/mnfr.202100260
DO - 10.1002/mnfr.202100260
M3 - Journal article
C2 - 35072987
VL - 66
JO - Molecular Nutrition & Food Research
JF - Molecular Nutrition & Food Research
SN - 1613-4125
IS - 6
M1 - 2100260
ER -
ID: 290667439