The removal of protein from mineral surfaces: Implications for residue analysis of archaeological materials
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The removal of protein from mineral surfaces : Implications for residue analysis of archaeological materials. / Craig, Oliver E.; Collins, Matthew J.
In: Journal of Archaeological Science, Vol. 29, No. 10, 2002, p. 1077-1082.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The removal of protein from mineral surfaces
T2 - Implications for residue analysis of archaeological materials
AU - Craig, Oliver E.
AU - Collins, Matthew J.
PY - 2002
Y1 - 2002
N2 - Although there has been great interest and controversy concerning the detection of proteins in archaeological residues, very few studies have considered the effect of different extraction methods. Using well-defined control materials, this study compares the extraction efficiency of a number of different extraction reagents. Immunological and spectro-photometric assays are used to assess the amount of protein removed by each extraction method and the effectiveness is reported with reference to previous work. None of the previously reported methods were found to be effective at removing the protein from the mineral surface. We conclude that proteins are very strongly bound to the surface probably via short-range bonds. Although this may be encouraging for the long-term preservation of proteins in this context, extraction methods will have to be re-evaluated in order to achieve efficient recovery.
AB - Although there has been great interest and controversy concerning the detection of proteins in archaeological residues, very few studies have considered the effect of different extraction methods. Using well-defined control materials, this study compares the extraction efficiency of a number of different extraction reagents. Immunological and spectro-photometric assays are used to assess the amount of protein removed by each extraction method and the effectiveness is reported with reference to previous work. None of the previously reported methods were found to be effective at removing the protein from the mineral surface. We conclude that proteins are very strongly bound to the surface probably via short-range bonds. Although this may be encouraging for the long-term preservation of proteins in this context, extraction methods will have to be re-evaluated in order to achieve efficient recovery.
KW - Ancient proteins
KW - Elisa
KW - Mineral surface
KW - Residue analysis
KW - Sorption experiments
U2 - 10.1006/jasc.2001.0757
DO - 10.1006/jasc.2001.0757
M3 - Journal article
AN - SCOPUS:0036786128
VL - 29
SP - 1077
EP - 1082
JO - Journal of Archaeological Science
JF - Journal of Archaeological Science
SN - 0305-4403
IS - 10
ER -
ID: 232090517