Single-walled carbon nanotube surface control of complement recognition and activation
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Single-walled carbon nanotube surface control of complement recognition and activation. / Andersen, Alina Joukainen; Robinson, Joshua T; Dai, Hongjie; Hunter, A Christy; Andresen, Thomas L; Moghimi, Seyed Moien.
In: A C S Nano, Vol. 7, No. 2, 26.02.2013, p. 1108-19.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Single-walled carbon nanotube surface control of complement recognition and activation
AU - Andersen, Alina Joukainen
AU - Robinson, Joshua T
AU - Dai, Hongjie
AU - Hunter, A Christy
AU - Andresen, Thomas L
AU - Moghimi, Seyed Moien
PY - 2013/2/26
Y1 - 2013/2/26
N2 - Carbon nanotubes (CNTs) are receiving considerable attention in site-specific drug and nucleic acid delivery, photodynamic therapy, and photoacoustic molecular imaging. Despite these advances, nanotubes may activate the complement system (an integral part of innate immunity), which can induce clinically significant anaphylaxis. We demonstrate that single-walled CNTs coated with human serum albumin activate the complement system through C1q-mediated classical and the alternative pathways. Surface coating with methoxypoly(ethylene glycol)-based amphiphiles, which confers solubility and prolongs circulation profiles of CNTs, activates the complement system differently, depending on the amphiphile structure. CNTs with linear poly(ethylene glycol) amphiphiles trigger the lectin pathway of the complement through both L-ficolin and mannan-binding lectin recognition. The lectin pathway activation, however, did not trigger the amplification loop of the alternative pathway. An amphiphile with branched poly(ethylene glycol) architecture also activated the lectin pathway but only through L-ficolin recognition. Importantly, this mode of activation neither generated anaphylatoxins nor induced triggering of the effector arm of the complement system. These observations provide a major step toward nanomaterial surface modification with polymers that have the properties to significantly improve innate immunocompatibility by limiting the formation of complement C3 and C5 convertases.
AB - Carbon nanotubes (CNTs) are receiving considerable attention in site-specific drug and nucleic acid delivery, photodynamic therapy, and photoacoustic molecular imaging. Despite these advances, nanotubes may activate the complement system (an integral part of innate immunity), which can induce clinically significant anaphylaxis. We demonstrate that single-walled CNTs coated with human serum albumin activate the complement system through C1q-mediated classical and the alternative pathways. Surface coating with methoxypoly(ethylene glycol)-based amphiphiles, which confers solubility and prolongs circulation profiles of CNTs, activates the complement system differently, depending on the amphiphile structure. CNTs with linear poly(ethylene glycol) amphiphiles trigger the lectin pathway of the complement through both L-ficolin and mannan-binding lectin recognition. The lectin pathway activation, however, did not trigger the amplification loop of the alternative pathway. An amphiphile with branched poly(ethylene glycol) architecture also activated the lectin pathway but only through L-ficolin recognition. Importantly, this mode of activation neither generated anaphylatoxins nor induced triggering of the effector arm of the complement system. These observations provide a major step toward nanomaterial surface modification with polymers that have the properties to significantly improve innate immunocompatibility by limiting the formation of complement C3 and C5 convertases.
KW - Complement C3
KW - Complement System Proteins
KW - Humans
KW - Hydrophobic and Hydrophilic Interactions
KW - Lectins
KW - Nanotubes, Carbon
KW - Polyethylene Glycols
KW - Protein Binding
KW - Serum Albumin
KW - Surface Properties
U2 - 10.1021/nn3055175
DO - 10.1021/nn3055175
M3 - Journal article
C2 - 23301860
VL - 7
SP - 1108
EP - 1119
JO - A C S Nano
JF - A C S Nano
SN - 1936-0851
IS - 2
ER -
ID: 106534979