Sequence analysis of three mitochondrial DNA molecules reveals interesting differences among Saccharomyces yeasts
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Sequence analysis of three mitochondrial DNA molecules reveals interesting differences among Saccharomyces yeasts. / Langkjaer, R B; Casaregola, S; Ussery, D W; Gaillardin, C; Piskur, J.
In: Nucleic Acids Research, Vol. 31, No. 12, 2003, p. 3081-91.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Sequence analysis of three mitochondrial DNA molecules reveals interesting differences among Saccharomyces yeasts
AU - Langkjaer, R B
AU - Casaregola, S
AU - Ussery, D W
AU - Gaillardin, C
AU - Piskur, J
N1 - Keywords: Base Sequence; DNA, Intergenic; DNA, Mitochondrial; Endodeoxyribonucleases; Endoribonucleases; Gene Order; Genes, rRNA; Introns; Mitochondrial Proteins; Molecular Sequence Data; Open Reading Frames; RNA; RNA, Catalytic; RNA, Transfer; Repetitive Sequences, Nucleic Acid; Ribonuclease P; Saccharomyces; Saccharomyces cerevisiae; Sequence Analysis, DNA; Species Specificity; Transcription Initiation Site; Transcription, Genetic
PY - 2003
Y1 - 2003
N2 - The complete sequences of mitochondrial DNA (mtDNA) from the two budding yeasts Saccharomyces castellii and Saccharomyces servazzii, consisting of 25 753 and 30 782 bp, respectively, were analysed and compared to Saccharomyces cerevisiae mtDNA. While some of the traits are very similar among Saccharomyces yeasts, others have highly diverged. The two mtDNAs are much more compact than that of S.cerevisiae and contain fewer introns and intergenic sequences, although they have almost the same coding potential. A few genes contain group I introns, but group II introns, otherwise found in S.cerevisiae mtDNA, are not present. Surprisingly, four genes (ATP6, COX2, COX3 and COB) in the mtDNA of S.servazzii contain, in total, five +1 frameshifts. mtDNAs of S.castellii, S.servazzii and S.cerevisiae contain all genes on the same strand, except for one tRNA gene. On the other hand, the gene order is very different. Several gene rearrangements have taken place upon separation of the Saccharomyces lineages, and even a part of the transcription units have not been preserved. It seems that the mechanism(s) involved in the generation of the rearrangements has had to ensure that all genes stayed encoded by the same DNA strand.
AB - The complete sequences of mitochondrial DNA (mtDNA) from the two budding yeasts Saccharomyces castellii and Saccharomyces servazzii, consisting of 25 753 and 30 782 bp, respectively, were analysed and compared to Saccharomyces cerevisiae mtDNA. While some of the traits are very similar among Saccharomyces yeasts, others have highly diverged. The two mtDNAs are much more compact than that of S.cerevisiae and contain fewer introns and intergenic sequences, although they have almost the same coding potential. A few genes contain group I introns, but group II introns, otherwise found in S.cerevisiae mtDNA, are not present. Surprisingly, four genes (ATP6, COX2, COX3 and COB) in the mtDNA of S.servazzii contain, in total, five +1 frameshifts. mtDNAs of S.castellii, S.servazzii and S.cerevisiae contain all genes on the same strand, except for one tRNA gene. On the other hand, the gene order is very different. Several gene rearrangements have taken place upon separation of the Saccharomyces lineages, and even a part of the transcription units have not been preserved. It seems that the mechanism(s) involved in the generation of the rearrangements has had to ensure that all genes stayed encoded by the same DNA strand.
M3 - Journal article
C2 - 12799436
VL - 31
SP - 3081
EP - 3091
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 12
ER -
ID: 14304067