Selection of functional tRNA primers and primer binding site sequences from a retroviral combinatorial library: identification of new functional tRNA primers in murine leukemia virus replication.
Research output: Contribution to journal › Journal article › Research › peer-review
Retroviral reverse transcription is initiated from a cellular tRNA molecule and all known exogenous isolates of murine leukemia virus utilise a tRNA(Pro)molecule. While several studies suggest flexibility in murine leukemia virus primer utilisation, studies on human immunodeficiency virus and avian retro-viruses have revealed evidence of molecular adapt-ation towards the specific tRNA isoacceptor used as replication primer. In this study, murine leukemia virus tRNA utilisation is investigated by in vivo screening of a retroviral vector combinatorial library with randomised primer binding sites. While most of the selected primer binding sites are complementary to the 3'-end of tRNA((Pro)), we also retrieved PBS sequences matching four other tRNA molecules and demonstrate that Akv murine leukemia virus vectors may efficiently replicate using tRNA(Arg(CCU)), tRNA(Phe(GAA))and a hitherto unknown human tRNA(Ser(CGA)).
| Original language | English |
|---|---|
| Journal | Nucleic Acids Research |
| Volume | 28 |
| Issue number | 3 |
| Pages (from-to) | 791-9 |
| Number of pages | 8 |
| ISSN | 0305-1048 |
| Publication status | Published - 2000 |
Bibliographical note
Keywords: 3T3 Cells; Animals; Base Sequence; Bias (Epidemiology); Binding Sites; Blotting, Northern; Cell Line; Gene Library; Genetic Vectors; Humans; Leukemia Virus, Murine; Mice; Nucleic Acid Hybridization; Polymerase Chain Reaction; RNA; RNA, Transfer; RNA, Transfer, Arg; RNA, Transfer, Phe; RNA, Transfer, Ser; Random Allocation; Regulatory Sequences, Nucleic Acid; Substrate Specificity; Virus Replication
ID: 5016425