Microscale purification of proteins by line immunoelectrophoresis: application of the technique in protein biogenesis studies
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A small-scale version of line immunoelectrophoresis in combination with immunoprecipitate excision is described as a rapid and convenient technique to purify proteins on a micro scale in biogenesis studies. In the purification and to result in a higher state of purity than an isolation procedure using protein A-Sepharose. Since the method furthermore allows a simultaneous purification of several different protein antigens from the same sample, it may be of interest as an alternative method to other procedures in the purification of proteins on a micro scale.
Original language | English |
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Journal | Journal of Proteomics |
Volume | 8 |
Issue number | 1 |
Pages (from-to) | 41-7 |
Number of pages | 6 |
ISSN | 1874-3919 |
Publication status | Published - 1983 |
Bibliographical note
Keywords: Animals; Immunoelectrophoresis; Intestine, Small; Microvilli; Protein Biosynthesis; Proteins; Swine
ID: 9881450