Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Measuring the kinase activity of the 5'-AMP-activated protein kinase (AMPK) is an essential part of understanding the regulation of this metabolic master switch. The AMPK heterotrimer can exist in 12 different constellations with potentially diverse activation patterns. It is therefore important to be able to measure heterotrimer-specific activity to discriminate between these patterns. In this chapter we describe how to measure the AMPK activity of specific heterotrimeric complexes by consecutive immunoprecipitations and how the assay can be performed in a medium throughput fashion using 96-well plates.
Original language | English |
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Title of host publication | AMPK : Methods and Protecols |
Editors | Dietbert Neumann, Benoit Viollet |
Number of pages | 14 |
Place of Publication | New York |
Publisher | Humana Press |
Publication date | 2018 |
Pages | 215-228 |
Chapter | 14 |
ISBN (Print) | 978-1-4939-7597-6 |
ISBN (Electronic) | 978-1-4939-7598-3 |
DOIs | |
Publication status | Published - 2018 |
Series | Methods in Molecular Biology |
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Number | 1732 |
ISSN | 1064-3745 |
- Faculty of Science - Immunoprecipitation, AMPK activity, Skeletal muscle, Isoform-specific, Radioactive tracer 33P, AMARA peptide
Research areas
ID: 191294168