Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF
Research output: Contribution to journal › Journal article › Research › peer-review
A fluorescein-conjugated bioactive analog of corticotropin-releasing factor (CRF) was synthesized and used to label cells that have high affinity CRF-binding sites. Of cultured bovine anterior pituitary cells, 6.1 +/- 0.6% were visible by fluorescence microscopy after incubation with the analog. Fluorescence was eliminated by coincubation with a 200-fold excess of unlabeled CRF. Treatment with dexamethasone (10(-9)-10(-7) M) decreased visible fluorescence in a dose-dependent manner. These results demonstrate the utility of a fluorescent CRF analog for identification of cells with specific CRF-binding sites and suggest that binding of CRF to anterior pituitary cells is altered by glucocorticoids.
Original language | English |
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Journal | Endocrinology |
Volume | 119 |
Issue number | 5 |
Pages (from-to) | 2376-82 |
Number of pages | 7 |
ISSN | 0013-7227 |
DOIs | |
Publication status | Published - Nov 1986 |
- Adrenocorticotropic Hormone, Animals, Cattle, Corticotropin-Releasing Hormone, Dexamethasone, Dose-Response Relationship, Drug, Flow Cytometry, Fluorescent Dyes, Microscopy, Fluorescence, Pituitary Gland, Anterior, Rats
Research areas
ID: 132900851