Hemolytic plate assay for quantification of active human complement component C3 using methylamine-treated plasma as complement source

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Hemolytic plate assay for quantification of active human complement component C3 using methylamine-treated plasma as complement source. / Ploug, M; Jessen, T E; Welinder, K. G.; Barkholt, V.

In: Analytical Biochemistry, Vol. 146, No. 2, 01.05.1985, p. 411-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ploug, M, Jessen, TE, Welinder, KG & Barkholt, V 1985, 'Hemolytic plate assay for quantification of active human complement component C3 using methylamine-treated plasma as complement source', Analytical Biochemistry, vol. 146, no. 2, pp. 411-7.

APA

Ploug, M., Jessen, T. E., Welinder, K. G., & Barkholt, V. (1985). Hemolytic plate assay for quantification of active human complement component C3 using methylamine-treated plasma as complement source. Analytical Biochemistry, 146(2), 411-7.

Vancouver

Ploug M, Jessen TE, Welinder KG, Barkholt V. Hemolytic plate assay for quantification of active human complement component C3 using methylamine-treated plasma as complement source. Analytical Biochemistry. 1985 May 1;146(2):411-7.

Author

Ploug, M ; Jessen, T E ; Welinder, K. G. ; Barkholt, V. / Hemolytic plate assay for quantification of active human complement component C3 using methylamine-treated plasma as complement source. In: Analytical Biochemistry. 1985 ; Vol. 146, No. 2. pp. 411-7.

Bibtex

@article{d5118904fe044343801751aa7b601b11,
title = "Hemolytic plate assay for quantification of active human complement component C3 using methylamine-treated plasma as complement source",
abstract = "A hemolytic plate assay specific for active human complement component C3 is described. The method is well suited for tracing active C3 during preparative purification or for screening of plasma samples. The assay is based on activation of the alternative pathway of complement by unmodified rabbit erythrocytes. Plasma treated with methylamine supplies the essential complement components other than C3. The lytic reaction is complete in 5 h at 37 degrees C and is unchanged by incubation overnight. The dose-response curve, i.e., lysis diameter versus logarithm of C3 concentration, is linear within 0.1-10 times normal plasma concentrations of C3. The standard deviation is below 10%. The hemolytic agarose plates are easy and inexpensive to prepare, and they can be stored at 4 degrees C for 2 weeks before use. This paper describes the optimal conditions of the assay and proves its specificity. Its use in C3 preparation and plasma screening for C3 is discussed.",
keywords = "Animals, Chemical Precipitation, Complement C3, Dose-Response Relationship, Immunologic, Edetic Acid, Erythrocytes, Hemolytic Plaque Technique, Humans, Magnesium, Methylamines, Rabbits, Sepharose, Journal Article",
author = "M Ploug and Jessen, {T E} and Welinder, {K. G.} and V. Barkholt",
year = "1985",
month = may,
day = "1",
language = "English",
volume = "146",
pages = "411--7",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Hemolytic plate assay for quantification of active human complement component C3 using methylamine-treated plasma as complement source

AU - Ploug, M

AU - Jessen, T E

AU - Welinder, K. G.

AU - Barkholt, V.

PY - 1985/5/1

Y1 - 1985/5/1

N2 - A hemolytic plate assay specific for active human complement component C3 is described. The method is well suited for tracing active C3 during preparative purification or for screening of plasma samples. The assay is based on activation of the alternative pathway of complement by unmodified rabbit erythrocytes. Plasma treated with methylamine supplies the essential complement components other than C3. The lytic reaction is complete in 5 h at 37 degrees C and is unchanged by incubation overnight. The dose-response curve, i.e., lysis diameter versus logarithm of C3 concentration, is linear within 0.1-10 times normal plasma concentrations of C3. The standard deviation is below 10%. The hemolytic agarose plates are easy and inexpensive to prepare, and they can be stored at 4 degrees C for 2 weeks before use. This paper describes the optimal conditions of the assay and proves its specificity. Its use in C3 preparation and plasma screening for C3 is discussed.

AB - A hemolytic plate assay specific for active human complement component C3 is described. The method is well suited for tracing active C3 during preparative purification or for screening of plasma samples. The assay is based on activation of the alternative pathway of complement by unmodified rabbit erythrocytes. Plasma treated with methylamine supplies the essential complement components other than C3. The lytic reaction is complete in 5 h at 37 degrees C and is unchanged by incubation overnight. The dose-response curve, i.e., lysis diameter versus logarithm of C3 concentration, is linear within 0.1-10 times normal plasma concentrations of C3. The standard deviation is below 10%. The hemolytic agarose plates are easy and inexpensive to prepare, and they can be stored at 4 degrees C for 2 weeks before use. This paper describes the optimal conditions of the assay and proves its specificity. Its use in C3 preparation and plasma screening for C3 is discussed.

KW - Animals

KW - Chemical Precipitation

KW - Complement C3

KW - Dose-Response Relationship, Immunologic

KW - Edetic Acid

KW - Erythrocytes

KW - Hemolytic Plaque Technique

KW - Humans

KW - Magnesium

KW - Methylamines

KW - Rabbits

KW - Sepharose

KW - Journal Article

M3 - Journal article

C2 - 3927773

VL - 146

SP - 411

EP - 417

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -

ID: 178214553