Demonstration of immunochemical identity between the nerve growth factor-inducible large external (NILE) glycoprotein and the cell adhesion molecule L1
Research output: Contribution to journal › Journal article › Research › peer-review
The nerve growth factor-inducible large external (NILE) glycoprotein and the neural cell adhesion molecule L1 were shown to be immunochemically identical. Immunoprecipitation with L1 and NILE antibodies of [3H]fucose-labeled material from culture supernatants and detergent extracts of NGF-treated rat PC12 pheochromocytoma cells yielded comigrating bands by SDS-PAGE. NILE antibodies reacted with immunopurified L1 antigen, but not with N-CAM and other L2 epitope-bearing glycoproteins from adult mouse brain. Finally, by sequential immunoprecipitation from detergent extracts of [35S]methionine-labeled early post-natal cerebellar cell cultures or [3H]fucose-labeled NGF-treated PC12 cells, all immunoreactivity for NILE antibody could be removed by pre-clearing with L1 antibody and vice versa.
| Original language | English |
|---|---|
| Journal | EMBO Journal |
| Volume | 4 |
| Issue number | 11 |
| Pages (from-to) | 2765-8 |
| Number of pages | 3 |
| ISSN | 0261-4189 |
| Publication status | Published - 1985 |
Bibliographical note
Keywords: Adrenal Gland Neoplasms; Animals; Antibodies; Antigen-Antibody Complex; Antigens, Surface; Cell Adhesion Molecules; Cell Line; Cells, Cultured; Cerebellum; Epitopes; Fucose; Glycoproteins; Immunoassay; Methionine; Mice; Mice, Inbred C57BL; Molecular Weight; Nerve Growth Factors; Neural Cell Adhesion Molecule L1; Pheochromocytoma; Rats; Sulfur Radioisotopes; Tritium
ID: 21607137